In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition
Rodriguez, F., Popolo, L., Vai, M., Lacanà, E., & Alberghina, L. (1990). Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae. EXPERIMENTAL CELL RESEARCH, 187(2), 315-319 [10.1016/0014-4827(90)90097-T].
Citazione: | Rodriguez, F., Popolo, L., Vai, M., Lacanà, E., & Alberghina, L. (1990). Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae. EXPERIMENTAL CELL RESEARCH, 187(2), 315-319 [10.1016/0014-4827(90)90097-T]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae | |
Autori: | Rodriguez, F; Popolo, L; Vai, M; Lacanà, E; Alberghina, L | |
Autori: | ALBERGHINA, LILIA (Ultimo) | |
Data di pubblicazione: | 1990 | |
Lingua: | English | |
Rivista: | EXPERIMENTAL CELL RESEARCH | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0014-4827(90)90097-T | |
Appare nelle tipologie: | 01 - Articolo su rivista |