In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition

Rodriguez, F., Popolo, L., Vai, M., Lacanà, E., & Alberghina, L. (1990). Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae. EXPERIMENTAL CELL RESEARCH, 187(2), 315-319 [10.1016/0014-4827(90)90097-T].

Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae

VAI, MARINA;ALBERGHINA, LILIA
Ultimo
1990

Abstract

In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition
Articolo in rivista - Articolo scientifico
Two-Dimensional Electrophoresis, Saccharomyces cerevisiae
English
315
319
5
Rodriguez, F., Popolo, L., Vai, M., Lacanà, E., & Alberghina, L. (1990). Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae. EXPERIMENTAL CELL RESEARCH, 187(2), 315-319 [10.1016/0014-4827(90)90097-T].
Rodriguez, F; Popolo, L; Vai, M; Lacanà, E; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/98158
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