Purpose: Tear fluid contains antioxidative compounds, vitamin C, glutathione, superoxide dismutase, and lactoferrin (LF), which protect the corneal epithelium from the effects of ultraviolet irradiation, direct airflow, and chemical agents. However, these natural defenses against oxidative stress can decrease, favoring the development of anterior eye disorders, such as keratoconus, dry eye, and Sjögren syndrome. LF is an iron-binding glycoprotein, present in mammalian secretions such as tears and milk, endowed with different physiological functions such as antimicrobial, antiviral, and antioxidant activities. In this work, we studied the capability of different soft contact lenses to adsorb and release LF to restore cellular viability in oxidative stress conditions. Methods: Three types of contact lenses (filcon V, galyfilcon A, and filcon IB) were loaded with LF and then incubated with TsA or human corneal epithelial primary cells. After oxidative stress induction with 250 M or 125 M H 2 O 2, cell viability was evaluated. Results: Data showed that the highest quantity of LF loaded in contact lenses was between 61 g (for filcon V) and 39 g (for filcon IB); the release was between 49% and 100% of protein adsorbed. LF released from contact lenses maintained its antioxidant activity at least for 24 hours and was able to protect human epithelial cells from the detrimental effects of oxidative stress. Conclusions: These results demonstrate that LF-loaded contact lenses could represent a new therapeutic approach to treat ocular surface pathologies characterized by high levels of oxidative stress.

Pastori, V., Tavazzi, S., Lecchi, M. (2015). Lactoferrin-loaded contact lenses: Eye protection against oxidative stress. CORNEA, 34(6), 693-697 [10.1097/ICO.0000000000000435].

Lactoferrin-loaded contact lenses: Eye protection against oxidative stress

PASTORI, VALENTINA
;
TAVAZZI, SILVIA
Secondo
;
LECCHI, MARZIA MARIA
Ultimo
2015

Abstract

Purpose: Tear fluid contains antioxidative compounds, vitamin C, glutathione, superoxide dismutase, and lactoferrin (LF), which protect the corneal epithelium from the effects of ultraviolet irradiation, direct airflow, and chemical agents. However, these natural defenses against oxidative stress can decrease, favoring the development of anterior eye disorders, such as keratoconus, dry eye, and Sjögren syndrome. LF is an iron-binding glycoprotein, present in mammalian secretions such as tears and milk, endowed with different physiological functions such as antimicrobial, antiviral, and antioxidant activities. In this work, we studied the capability of different soft contact lenses to adsorb and release LF to restore cellular viability in oxidative stress conditions. Methods: Three types of contact lenses (filcon V, galyfilcon A, and filcon IB) were loaded with LF and then incubated with TsA or human corneal epithelial primary cells. After oxidative stress induction with 250 M or 125 M H 2 O 2, cell viability was evaluated. Results: Data showed that the highest quantity of LF loaded in contact lenses was between 61 g (for filcon V) and 39 g (for filcon IB); the release was between 49% and 100% of protein adsorbed. LF released from contact lenses maintained its antioxidant activity at least for 24 hours and was able to protect human epithelial cells from the detrimental effects of oxidative stress. Conclusions: These results demonstrate that LF-loaded contact lenses could represent a new therapeutic approach to treat ocular surface pathologies characterized by high levels of oxidative stress.
Articolo in rivista - Articolo scientifico
contact lens; cornea; lactoferrin; oxidative stress; viability test; Anti-Infective Agents; Apoproteins; Cell Line; Cell Survival; Corneal Diseases; Epithelium, Corneal; Humans; Hydrogen Peroxide; Lactoferrin; Oxidants; Oxidative Stress; Contact Lenses, Hydrophilic; Drug Delivery Systems; Ophthalmology
English
693
697
5
Pastori, V., Tavazzi, S., Lecchi, M. (2015). Lactoferrin-loaded contact lenses: Eye protection against oxidative stress. CORNEA, 34(6), 693-697 [10.1097/ICO.0000000000000435].
Pastori, V; Tavazzi, S; Lecchi, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/98131
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