OBJECTIVES: We evaluated the possibility that a pattern of abnormal microRNA (miRNA) expression could be fuelling the mechanisms causing HIV-associated lipodystrophy (HAL).METHODS: In this case-control study, samples of subcutaneous adipose tissue from eight consecutive HIV-infected patients on combination antiretroviral therapy with HAL (cases) were compared with those of eight HIV-negative subjects (controls). Human miRNA microarrays were used to probe the transcriptomes of the samples. Analysis of differentially expressed miRNAs was performed using DataAssist v2.0 software, applying a paired Student's t-test.RESULTS: Data showed that 21 miRNAs out of 754 were overexpressed in the patient group. Ten of these (i.e. miR-186, miR-199a-3p, miR-214, miR-374a, miR-487b, miR-532-5p, miR-628-5p, miR-874, miR-125-b-1* and miR-374b*) were up-regulated to a significant degree (fold change >2.5; P < 0.01). Eleven other miRNAs (i.e. miR-let-7d, miR-24, miR-30c, miR-125a-3p, miR-149, miR-191, miR-196-b, miR-218, miR-342-3p, miR-452 and miR-454*) were 2- to 2.5-fold more expressed in HIV+ samples than in controls. Levels of mRNA for lipin 1, the target of miR-218, were significantly lower in subcutaneous adipose tissue from HIV patients.CONCLUSIONS: In adipocytes of HIV-infected patients, the up-regulation of specific miRNAs could lead to an increased 'activation' that might contribute to the pathogenesis of HAL by increasing cell turnover and/or promotion of apoptosis.

Squillace, N., Bresciani, E., Torsello, A., Bandera, A., Sabbatini, F., Giovannetti, C., et al. (2014). Changes in subcutaneous adipose tissue microRNA expression in HIV-infected patients. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 69(11), 3067-3075 [10.1093/jac/dku264].

Changes in subcutaneous adipose tissue microRNA expression in HIV-infected patients

SQUILLACE, NICOLA
Primo
;
BRESCIANI, ELENA
Secondo
;
TORSELLO, ANTONIO BIAGIO;BANDERA, ALESSANDRA;LOCATELLI, VITTORIO
Penultimo
;
GORI, ANDREA
Ultimo
2014

Abstract

OBJECTIVES: We evaluated the possibility that a pattern of abnormal microRNA (miRNA) expression could be fuelling the mechanisms causing HIV-associated lipodystrophy (HAL).METHODS: In this case-control study, samples of subcutaneous adipose tissue from eight consecutive HIV-infected patients on combination antiretroviral therapy with HAL (cases) were compared with those of eight HIV-negative subjects (controls). Human miRNA microarrays were used to probe the transcriptomes of the samples. Analysis of differentially expressed miRNAs was performed using DataAssist v2.0 software, applying a paired Student's t-test.RESULTS: Data showed that 21 miRNAs out of 754 were overexpressed in the patient group. Ten of these (i.e. miR-186, miR-199a-3p, miR-214, miR-374a, miR-487b, miR-532-5p, miR-628-5p, miR-874, miR-125-b-1* and miR-374b*) were up-regulated to a significant degree (fold change >2.5; P < 0.01). Eleven other miRNAs (i.e. miR-let-7d, miR-24, miR-30c, miR-125a-3p, miR-149, miR-191, miR-196-b, miR-218, miR-342-3p, miR-452 and miR-454*) were 2- to 2.5-fold more expressed in HIV+ samples than in controls. Levels of mRNA for lipin 1, the target of miR-218, were significantly lower in subcutaneous adipose tissue from HIV patients.CONCLUSIONS: In adipocytes of HIV-infected patients, the up-regulation of specific miRNAs could lead to an increased 'activation' that might contribute to the pathogenesis of HAL by increasing cell turnover and/or promotion of apoptosis.
Articolo in rivista - Articolo scientifico
adipose tissue abnormalities; HIV-associated lipodystrophy; miRNAs; Adult; Aged; Antiretroviral Therapy, Highly Active; Case-Control Studies; Gene Expression Regulation; HIV Infections; Humans; Male; MicroRNAs; Middle Aged; Subcutaneous Fat; Medicine (all)
English
2014
69
11
3067
3075
none
Squillace, N., Bresciani, E., Torsello, A., Bandera, A., Sabbatini, F., Giovannetti, C., et al. (2014). Changes in subcutaneous adipose tissue microRNA expression in HIV-infected patients. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 69(11), 3067-3075 [10.1093/jac/dku264].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/96821
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