A cultural system, aimed at the production of human interleukin-1β (IL-1β) with cells of a non-conventional yeast transformed for interleukin expression, Zygosaccharomyces bailii [pZ3KlIL-1β], was realized. Interleukin production was accomplished in a reactor operating in fed-batch mode to avoid sugar overflow metabolism, limitations with respect to oxygen transfer, and achieve high cell density. Batch operation mode was employed only to characterise the producer strain and experimentally estimate kinetic parameters. In parallel with strain characterisation, a mathematical model was developed. The comparison between simulations and experimental data allowed to evidence the importance of physiological state of inoculum, being only a fermentative one suitable to sustain a given exponential growth. The respiratory capacity of Z. bailii [pZ3KlIL-1β], resulted to be affected by stirring. The theoretical and experimental approach allowed the bioprocess optimisation. © 2009 Elsevier Ltd. All rights reserved.
Romano, V., Paciello, L., Romano, F., de Alteriis, E., Brambilla, L.G., & Parascandola, P. (2009). Interleukin-1[beta] production by Zygosaccharomyces bailii [pZ3 KlIL-1[beta]] in aerated fed-batch reactor: Importance of inoculum physiology and bioprocess modelling. PROCESS BIOCHEMISTRY, 44(5), 527-533 [10.1016/j.procbio.2009.01.004].
Citazione: | Romano, V., Paciello, L., Romano, F., de Alteriis, E., Brambilla, L.G., & Parascandola, P. (2009). Interleukin-1[beta] production by Zygosaccharomyces bailii [pZ3 KlIL-1[beta]] in aerated fed-batch reactor: Importance of inoculum physiology and bioprocess modelling. PROCESS BIOCHEMISTRY, 44(5), 527-533 [10.1016/j.procbio.2009.01.004]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Titolo: | Interleukin-1[beta] production by Zygosaccharomyces bailii [pZ3 KlIL-1[beta]] in aerated fed-batch reactor: Importance of inoculum physiology and bioprocess modelling | |
Autori: | Romano, V; Paciello, L; Romano, F; de Alteriis, E; Brambilla, LG; Parascandola, P | |
Autori: | ||
Data di pubblicazione: | 2009 | |
Lingua: | English | |
Rivista: | PROCESS BIOCHEMISTRY | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.procbio.2009.01.004 | |
Appare nelle tipologie: | 01 - Articolo su rivista |