The focused ion beam (FIB) and scanning electron microscope (SEM) are commonly used in material sciences for imaging and analysis of materials. Over the last decade, the combined FIB/SEM system has proven to be also applicable in the life sciences. We have examined the potential of the focused ion beam/scanning electron microscope system for the investigation of biological tissues of the model organism Porcellio scaber (Crustacea: Isopoda). Tissue from digestive glands was prepared as for conventional SEM or as for transmission electron microscopy (TEM). The samples were transferred into FIB/SEM for FIB milling and an imaging operation. FIB-milled regions were secondary electron imaged, back-scattered electron imaged, or energy dispersive X-ray (EDX) analyzed. Our results demonstrated that FIB/SEM enables simultaneous investigation of sample gross morphology, cell surface characteristics, and subsurface structures. The same FIB-exposed regions were analyzed by EDX to provide basic compositional data. When samples were prepared as for TEM, the information obtained with FIB/SEM is comparable, though at limited magnification, to that obtained from TEM. A combination of imaging, micro-manipulation, and compositional analysis appears of particular interest in the investigation of epithelial tissues, which are subjected to various endogenous and exogenous conditions affecting their structure and function. The FIB/SEM is a promising tool for an overall examination of epithelial tissue under normal, stressed, or pathological conditions. © 2010 Springer-Verlag.
Lešer, V., Milani, M., Tatti, F., Tkalec, Z., , Š., Žnidaršič, N., et al. (2010). Contribution of FIB/SEM to tissue structural research. PROTOPLASMA, 2010-02 [10.1007/s00709-010-0118-8].
|Citazione:||Lešer, V., Milani, M., Tatti, F., Tkalec, Z., , Š., Žnidaršič, N., et al. (2010). Contribution of FIB/SEM to tissue structural research. PROTOPLASMA, 2010-02 [10.1007/s00709-010-0118-8].|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Titolo:||Contribution of FIB/SEM to tissue structural research|
|Autori:||Lešer, V; Milani, M; Tatti, F; Tkalec, Z;, Štrus, J; Žnidaršič, N; Drobne, D|
|Data di pubblicazione:||feb-2010|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00709-010-0118-8|
|Appare nelle tipologie:||01 - Articolo su rivista|