Accurate diagnosis of canine filariosis is essential for choosing correct therapeutic approach. Therefore, reliable methods for discriminating among the different filarial infections in dogs are needed. The authors report simple and highly specific molecular methods that identify the three most common filarial nematodes of European dogs: Dirofilaria immitis, D. repens and Acanthocheilonema (syn. Dipetalonema) reconditum, based on (1) PCR amplifications of mitochondrial DNA (12S rDNA and coxI) with general filarial primers followed by digestion with restriction enzymes that generates band polymorphisms clearly discriminating the three species and (2) PCR amplifications with species-specific primers to support the restriction analysis, in particular in the case of multiple infections. (c) 2006 Elsevier B.V. All rights reserved.

Casiraghi, M., Bazzocchi, C., Mortarino, M., Ottina, E., Genchi, C. (2006). A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris). VETERINARY PARASITOLOGY, 141(3-4), 368-372 [10.1016/j.vetpar.2006.06.006].

A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)

CASIRAGHI, MAURIZIO;
2006

Abstract

Accurate diagnosis of canine filariosis is essential for choosing correct therapeutic approach. Therefore, reliable methods for discriminating among the different filarial infections in dogs are needed. The authors report simple and highly specific molecular methods that identify the three most common filarial nematodes of European dogs: Dirofilaria immitis, D. repens and Acanthocheilonema (syn. Dipetalonema) reconditum, based on (1) PCR amplifications of mitochondrial DNA (12S rDNA and coxI) with general filarial primers followed by digestion with restriction enzymes that generates band polymorphisms clearly discriminating the three species and (2) PCR amplifications with species-specific primers to support the restriction analysis, in particular in the case of multiple infections. (c) 2006 Elsevier B.V. All rights reserved.
Articolo in rivista - Articolo scientifico
Dirofilaria immitis; Dirifilaria repens; Acanthocheilonema reconditum; PCR; restriction enzyme; mtDNA
English
5-nov-2006
141
3-4
368
372
none
Casiraghi, M., Bazzocchi, C., Mortarino, M., Ottina, E., Genchi, C. (2006). A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris). VETERINARY PARASITOLOGY, 141(3-4), 368-372 [10.1016/j.vetpar.2006.06.006].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/9112
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