Their physicochemical properties and relatively low cost make cellulose nanocrystals (CNCs) a potential candidate for future large-scale production in many fields including nanomedicine. Prior to a sustained and responsible development as theranostic agents, robust and reliable data concerning their safety, biocompatibility, and tissue distribution should be provided. In the present study, CNCs were extracted from Whatman filters functionalized with a fluorescent dye, and their interaction with living organisms has been thoroughly assessed. Our experimental evidence demonstrated that CNCs (1) are well tolerated by healthy mice after systemic injection; (2) are rapidly excreted, thus avoiding bioaccumulation in filter organs such as the kidneys and liver; (3) transiently migrate in bones; and (4) are able to penetrate in the cytoplasm of cancer cells without inducing material-related detrimental effects in terms of cell survival. Our results strongly suggest that the peculiar tropism to the bones is due to the chemical interaction between the Ca<sup>2+</sup> of the bone matrix and the active surface of negatively-charged CNCs. This feature, together with the ability to penetrate cancer cells, makes CNCs a potential nanodevice for theranostics in bone tumors.

Colombo, L., Zoia, L., Violatto, M., Previdi, S., Talamini, L., Sitia, L., et al. (2015). Organ distribution and bone tropism of cellulose nanocrystals in living mice. BIOMACROMOLECULES, 16(9), 2862-2871 [10.1021/acs.biomac.5b00805].

Organ distribution and bone tropism of cellulose nanocrystals in living mice

Zoia, L;Nicotra, F;Orlandi, M;La Ferla, B
2015

Abstract

Their physicochemical properties and relatively low cost make cellulose nanocrystals (CNCs) a potential candidate for future large-scale production in many fields including nanomedicine. Prior to a sustained and responsible development as theranostic agents, robust and reliable data concerning their safety, biocompatibility, and tissue distribution should be provided. In the present study, CNCs were extracted from Whatman filters functionalized with a fluorescent dye, and their interaction with living organisms has been thoroughly assessed. Our experimental evidence demonstrated that CNCs (1) are well tolerated by healthy mice after systemic injection; (2) are rapidly excreted, thus avoiding bioaccumulation in filter organs such as the kidneys and liver; (3) transiently migrate in bones; and (4) are able to penetrate in the cytoplasm of cancer cells without inducing material-related detrimental effects in terms of cell survival. Our results strongly suggest that the peculiar tropism to the bones is due to the chemical interaction between the Ca2+ of the bone matrix and the active surface of negatively-charged CNCs. This feature, together with the ability to penetrate cancer cells, makes CNCs a potential nanodevice for theranostics in bone tumors.
Articolo in rivista - Articolo scientifico
Biochemistry; Biocompatibility; Biology; Bone; Cells; Cellulose; Cytology; Diseases; Mammals; Medical nanotechnology; Nanocrystals
English
2862
2871
10
Colombo, L., Zoia, L., Violatto, M., Previdi, S., Talamini, L., Sitia, L., et al. (2015). Organ distribution and bone tropism of cellulose nanocrystals in living mice. BIOMACROMOLECULES, 16(9), 2862-2871 [10.1021/acs.biomac.5b00805].
File in questo prodotto:
File Dimensione Formato  
acs.biomac.5b00805.pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Dimensione 1.71 MB
Formato Adobe PDF
1.71 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/90012
Citazioni
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 55
Social impact