The biotechnological production of biodiesel is based on transesterification/esterification reactions between a source of fatty acids and a short-chain alcohol, usually methanol, catalysed by enzymes belonging to the class known as lipases. Several lipases used in industrial processes, although stable in the presence of other organic solvents, are inactivated by methanol at or below the concentration optimal for biodiesel production, making it necessary to use stepwise methanol feeding or pre-treatment of the enzyme. In this review article we focus on what is currently know about methanol inactivation of lipases, a phenomenon which is not common to all lipase enzymes, with the goal of improving the biocatalytic process. We suggest that different mechanisms can lead to inactivation of different lipases, in particular substrate inhibition and protein unfolding. Attempts to improve the performances of methanol sensitive lipases by mutagenesis as well as process engineering approaches are also summarized

Lotti, M., Pleiss, J., Valero, F., Ferrer, P. (2015). Effects of methanol on lipases: Molecular, kinetic and process issues in the production of biodiesel. BIOTECHNOLOGY JOURNAL, 10(1), 22-30 [10.1002/biot.201400158].

Effects of methanol on lipases: Molecular, kinetic and process issues in the production of biodiesel

Lotti, M
;
2015

Abstract

The biotechnological production of biodiesel is based on transesterification/esterification reactions between a source of fatty acids and a short-chain alcohol, usually methanol, catalysed by enzymes belonging to the class known as lipases. Several lipases used in industrial processes, although stable in the presence of other organic solvents, are inactivated by methanol at or below the concentration optimal for biodiesel production, making it necessary to use stepwise methanol feeding or pre-treatment of the enzyme. In this review article we focus on what is currently know about methanol inactivation of lipases, a phenomenon which is not common to all lipase enzymes, with the goal of improving the biocatalytic process. We suggest that different mechanisms can lead to inactivation of different lipases, in particular substrate inhibition and protein unfolding. Attempts to improve the performances of methanol sensitive lipases by mutagenesis as well as process engineering approaches are also summarized
Articolo in rivista - Articolo scientifico
Biodiesel; Inactivation; Lipase; Methanol
English
21-lug-2014
2015
10
1
22
30
partially_open
Lotti, M., Pleiss, J., Valero, F., Ferrer, P. (2015). Effects of methanol on lipases: Molecular, kinetic and process issues in the production of biodiesel. BIOTECHNOLOGY JOURNAL, 10(1), 22-30 [10.1002/biot.201400158].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/85225
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