Persistent infection with oncogenic human papillomavirus (HPV) is a prerequisite for cervical disease development, yet data regarding the host immune response to infection at the genotype level are quite limited. We created pseudoviruses bearing the major (L1) and minor (L2) capsid proteins and L1 virus-like particles representing the reference sequence and a consensus of 34 European sequences of HPV51. Despite the formation of similarly sized particles, motifs in the reference L1 and L2 genes had a profound impact on the immunogenicity, antigenicity and infectivity of these antigens. The antibody status of women exhibiting low-grade disease was similar between HPV16 and the consensus HPV51, but both demonstrated discrepancies between binding and neutralizing antibody responses. These data support the use of pseudoviruses as the preferred target antigen in studies of natural HPV infection and the need to consider variation in both the L1 and L2 proteins for the appropriate presentation of antibody epitopes.

Godi, A., Epifano, I., Bissett, S., Dell'Anna, T., Piana, A., Cocuzza, C., et al. (2015). Amino acid motifs in both the major and minor capsid proteins of HPV51 impact antigenicity and infectivity. JOURNAL OF GENERAL VIROLOGY, 96(7), 1842-1849 [10.1099/vir.0.000121].

Amino acid motifs in both the major and minor capsid proteins of HPV51 impact antigenicity and infectivity

COCUZZA, CLEMENTINA ELVEZIA
Primo
;
2015

Abstract

Persistent infection with oncogenic human papillomavirus (HPV) is a prerequisite for cervical disease development, yet data regarding the host immune response to infection at the genotype level are quite limited. We created pseudoviruses bearing the major (L1) and minor (L2) capsid proteins and L1 virus-like particles representing the reference sequence and a consensus of 34 European sequences of HPV51. Despite the formation of similarly sized particles, motifs in the reference L1 and L2 genes had a profound impact on the immunogenicity, antigenicity and infectivity of these antigens. The antibody status of women exhibiting low-grade disease was similar between HPV16 and the consensus HPV51, but both demonstrated discrepancies between binding and neutralizing antibody responses. These data support the use of pseudoviruses as the preferred target antigen in studies of natural HPV infection and the need to consider variation in both the L1 and L2 proteins for the appropriate presentation of antibody epitopes.
Articolo in rivista - Articolo scientifico
HPV51, capsid proteins, antigenicity, infectivity
English
2015
96
7
1842
1849
none
Godi, A., Epifano, I., Bissett, S., Dell'Anna, T., Piana, A., Cocuzza, C., et al. (2015). Amino acid motifs in both the major and minor capsid proteins of HPV51 impact antigenicity and infectivity. JOURNAL OF GENERAL VIROLOGY, 96(7), 1842-1849 [10.1099/vir.0.000121].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/81452
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