Homologous recombination requires nucleolytic degradation (resection) of DNA double-strand break (DSB) ends. In Saccharomyces cerevisiae, the MRX complex and Sae2 are involved in the onset of DSB resection, whereas extensive resection requires Exo1 and the concerted action of Dna2 and Sgs1. Here, we show that the checkpoint protein Rad9 limits the action of Sgs1/Dna2 in DSB resection by inhibiting Sgs1 binding/persistence at the DSB ends. When inhibition by Rad9 is abolished by the Sgs1-ss mutant variant or by deletion of RAD9, the requirement for Sae2 and functional MRX in DSB resection is reduced. These results provide new insights into how early and long-range resection is coordinated. Synopsis The checkpoint protein Rad9 inhibits the Sgs1/Dna2 long-range resection machinery and thereby increases the requirement for MRX/Sae2 activities in DSB resection. The Sgs1-ss mutant variant suppresses the sensitivity to DNA damaging agents and the resection defect of sae2 cells. Rad9 limits the action of Sgs1/Dna2 in DSB resection by inhibiting Sgs1 binding/persistence at the DSB ends. The escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DSB resection. The checkpoint protein Rad9 inhibits the Sgs1/Dna2 long-range resection machinery and thereby increases the requirement for MRX-Sae2 activities in DSB resection.
BONETTI, D., VILLA, M., GOBBINI, E., CASSANI, C., TEDESCHI, G., & LONGHESE, M. (2015). Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO REPORTS, 16(3), 351-361 [10.15252/embr.201439764].
Citazione: | BONETTI, D., VILLA, M., GOBBINI, E., CASSANI, C., TEDESCHI, G., & LONGHESE, M. (2015). Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO REPORTS, 16(3), 351-361 [10.15252/embr.201439764]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection | |
Autori: | BONETTI, D; VILLA, M; GOBBINI, E; CASSANI, C; TEDESCHI, G; LONGHESE, M | |
Autori: | BONETTI, DIEGO (Primo) VILLA, MATTEO (Secondo) LONGHESE, MARIA PIA (Ultimo) (Corresponding) | |
Data di pubblicazione: | 2015 | |
Lingua: | English | |
Rivista: | EMBO REPORTS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.15252/embr.201439764 | |
Appare nelle tipologie: | 01 - Articolo su rivista |
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