Diverse roles in DNA metabolism have been envisaged for budding yeast and mammalian Rif1. In particular, yeast Rif1 is involved in telomere homeostasis, while its mammalian counterpart participates in the cellular response to DNA double-strand breaks (DSBs). Here, we show that Saccharomyces cerevisiae Rif1 supports cell survival to DNA lesions in the absence of MRX or Sae2. Furthermore, it contributes to the nucleolytic processing (resection) of DSBs. This Rif1-dependent control of DSB resection becomes important for DSB repair by homologous recombination when resection activities are suboptimal. © 2014 The Authors.

Martina, M., Bonetti, D., Villa, M., Lucchini, G., & Longhese, M. (2014). Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. EMBO REPORTS, 15(6), 695-704 [10.1002/embr.201338338].

Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection

MARTINA, MARINA;BONETTI, DIEGO;VILLA, MATTEO;LUCCHINI, GIOVANNA;LONGHESE, MARIA PIA
2014

Abstract

Diverse roles in DNA metabolism have been envisaged for budding yeast and mammalian Rif1. In particular, yeast Rif1 is involved in telomere homeostasis, while its mammalian counterpart participates in the cellular response to DNA double-strand breaks (DSBs). Here, we show that Saccharomyces cerevisiae Rif1 supports cell survival to DNA lesions in the absence of MRX or Sae2. Furthermore, it contributes to the nucleolytic processing (resection) of DSBs. This Rif1-dependent control of DSB resection becomes important for DSB repair by homologous recombination when resection activities are suboptimal. © 2014 The Authors.
Articolo in rivista - Articolo scientifico
Scientifica
double-strand break; Rad9; resection; Rif1; Saccharomyces cerevisiae; Chromatin Immunoprecipitation; DNA Repair; DNA-Binding Proteins; Electrophoresis, Agar Gel; Endodeoxyribonucleases; Endonucleases; Exodeoxyribonucleases; Multiprotein Complexes; Repressor Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Telomere-Binding Proteins; DNA Breaks, Double-Stranded; Genetics; Molecular Biology; Biochemistry
English
Martina, M., Bonetti, D., Villa, M., Lucchini, G., & Longhese, M. (2014). Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. EMBO REPORTS, 15(6), 695-704 [10.1002/embr.201338338].
Martina, M; Bonetti, D; Villa, M; Lucchini, G; Longhese, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/80585
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