The epidemic of heart failure has stimulated interest in understanding cardiac regeneration. Evidence has been reported supporting regeneration via transplantation of multiple cell types, as well as replication of postmitotic cardiomyocytes. In addition, the adult myocardium harbors endogenous c-kit pos cardiac stem cells (eCSCs), whose relevance for regeneration is controversial. Here, using different rodent models of diffuse myocardial damage causing acute heart failure, we show that eCSCs restore cardiac function by regenerating lost cardiomyocytes. Ablation of the eCSC abolishes regeneration and functional recovery. The regenerative process is completely restored by replacing the ablated eCSCs with the progeny of one eCSC. eCSCs recovered from the host and recloned retain their regenerative potential in vivo and in vitro. After regeneration, selective suicide of these exogenous CSCs and their progeny abolishes regeneration, severely impairing ventricular performance. These data show that c-kitpos eCSCs are necessary and sufficient for the regeneration and repair of myocardial damage. © 2013 Elsevier Inc.

Ellison, G., Vicinanza, C., Smith, A., Aquila, I., Leone, A., Waring, C., et al. (2013). Adult c-kitpos cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair. CELL, 154(4), 827-842 [10.1016/j.cell.2013.07.039].

Adult c-kitpos cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair

CONDORELLI, GIANLUIGI;OTTOLENGHI, SERGIO;
2013

Abstract

The epidemic of heart failure has stimulated interest in understanding cardiac regeneration. Evidence has been reported supporting regeneration via transplantation of multiple cell types, as well as replication of postmitotic cardiomyocytes. In addition, the adult myocardium harbors endogenous c-kit pos cardiac stem cells (eCSCs), whose relevance for regeneration is controversial. Here, using different rodent models of diffuse myocardial damage causing acute heart failure, we show that eCSCs restore cardiac function by regenerating lost cardiomyocytes. Ablation of the eCSC abolishes regeneration and functional recovery. The regenerative process is completely restored by replacing the ablated eCSCs with the progeny of one eCSC. eCSCs recovered from the host and recloned retain their regenerative potential in vivo and in vitro. After regeneration, selective suicide of these exogenous CSCs and their progeny abolishes regeneration, severely impairing ventricular performance. These data show that c-kitpos eCSCs are necessary and sufficient for the regeneration and repair of myocardial damage. © 2013 Elsevier Inc.
Articolo in rivista - Articolo scientifico
Adult Stem Cells; Animals; Bone Marrow Cells; Green Fluorescent Proteins; Heart; Heart Failure; Humans; Isoproterenol; Male; Mice; Myocytes, Cardiac; Rats; Stem Cell Factor; Biochemistry, Genetics and Molecular Biology (all)
English
2013
154
4
827
842
none
Ellison, G., Vicinanza, C., Smith, A., Aquila, I., Leone, A., Waring, C., et al. (2013). Adult c-kitpos cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair. CELL, 154(4), 827-842 [10.1016/j.cell.2013.07.039].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/78145
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