Saccharomyces cerevisiae cells respond to hypotonic stress (HTS) by a cytosolic calcium rise, either generated by an influx of calcium from extracellular medium, when calcium is available, or by a release from intracellular stores in scarcity of extracellular calcium. Calcium release from intracellular compartments is peculiarly inhibited by external calcium in a calcineurin-independent and Cch1-, but not Mid1-, driven manner. HTS-induced calcium release is also negatively regulated by the ER protein Cls2 and involves a poorly characterized protein, FLC2/YAL053W gene product, previously proposed to be required for FAD transport in the ER, albeit, due to its molecular features, it was also previously classified as an ion transporter. A computational analysis revealed that this gene and its three homologs in S. cerevisiae, together with previously identified Schizosaccharomyces pombe pkd2 and Neurospora crassa calcium-related spray protein, belong to a fungal branch of TRP-like ion transporters related to human mucolipin and polycystin 2 calcium transporters. Moreover, disruption of FLC2 gene confers severe sensitivity to Calcofluor white and hyper-activation of the cell wall integrity MAPK cascade, suggesting a role in cell wall maintenance as previously suggested for the fission yeast homolog. Perturbation in cytosolic resting calcium concentration and hyper-activation of calcineurin in exponentially growing cells suggest a role for this transporter in calcium homeostasis in yeast.

Rigamonti, M., Groppi, S., Belotti, F., Ambrosini, R., Filippi, G., Martegani, E., et al. (2015). Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane. CELL CALCIUM, 57(2), 57-68 [10.1016/j.ceca.2014.12.003].

Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane

RIGAMONTI, MARCO
Primo
Membro del Collaboration Group
;
GROPPI, SILVIA
Secondo
Membro del Collaboration Group
;
BELOTTI, FIORELLA
Membro del Collaboration Group
;
AMBROSINI, ROBERTO;FILIPPI, GIULIA;MARTEGANI, ENZO
Penultimo
Membro del Collaboration Group
;
TISI, RENATA ANITA
Ultimo
Membro del Collaboration Group
2015

Abstract

Saccharomyces cerevisiae cells respond to hypotonic stress (HTS) by a cytosolic calcium rise, either generated by an influx of calcium from extracellular medium, when calcium is available, or by a release from intracellular stores in scarcity of extracellular calcium. Calcium release from intracellular compartments is peculiarly inhibited by external calcium in a calcineurin-independent and Cch1-, but not Mid1-, driven manner. HTS-induced calcium release is also negatively regulated by the ER protein Cls2 and involves a poorly characterized protein, FLC2/YAL053W gene product, previously proposed to be required for FAD transport in the ER, albeit, due to its molecular features, it was also previously classified as an ion transporter. A computational analysis revealed that this gene and its three homologs in S. cerevisiae, together with previously identified Schizosaccharomyces pombe pkd2 and Neurospora crassa calcium-related spray protein, belong to a fungal branch of TRP-like ion transporters related to human mucolipin and polycystin 2 calcium transporters. Moreover, disruption of FLC2 gene confers severe sensitivity to Calcofluor white and hyper-activation of the cell wall integrity MAPK cascade, suggesting a role in cell wall maintenance as previously suggested for the fission yeast homolog. Perturbation in cytosolic resting calcium concentration and hyper-activation of calcineurin in exponentially growing cells suggest a role for this transporter in calcium homeostasis in yeast.
Articolo in rivista - Articolo scientifico
Budding yeast; Mucolipin like calcium channels; Osmotic shock; PKD2 like calcium channels; Polycystin 2 like calcium channels; TRPML; TRPP; Cell Biology; Molecular Biology; Physiology
English
2015
57
2
57
68
partially_open
Rigamonti, M., Groppi, S., Belotti, F., Ambrosini, R., Filippi, G., Martegani, E., et al. (2015). Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane. CELL CALCIUM, 57(2), 57-68 [10.1016/j.ceca.2014.12.003].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/77641
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