Saccharomyces cerevisiae cells respond to hypotonic stress (HTS) by a cytosolic calcium rise, either generated by an influx of calcium from extracellular medium, when calcium is available, or by a release from intracellular stores in scarcity of extracellular calcium. Calcium release from intracellular compartments is peculiarly inhibited by external calcium in a calcineurin-independent and Cch1-, but not Mid1-, driven manner. HTS-induced calcium release is also negatively regulated by the ER protein Cls2 and involves a poorly characterized protein, FLC2/YAL053W gene product, previously proposed to be required for FAD transport in the ER, albeit, due to its molecular features, it was also previously classified as an ion transporter. A computational analysis revealed that this gene and its three homologs in S. cerevisiae, together with previously identified Schizosaccharomyces pombe pkd2 and Neurospora crassa calcium-related spray protein, belong to a fungal branch of TRP-like ion transporters related to human mucolipin and polycystin 2 calcium transporters. Moreover, disruption of FLC2 gene confers severe sensitivity to Calcofluor white and hyper-activation of the cell wall integrity MAPK cascade, suggesting a role in cell wall maintenance as previously suggested for the fission yeast homolog. Perturbation in cytosolic resting calcium concentration and hyper-activation of calcineurin in exponentially growing cells suggest a role for this transporter in calcium homeostasis in yeast.
RIGAMONTI, M., GROPPI, S., BELOTTI, F., AMBROSINI, R., FILIPPI, G., MARTEGANI, E., et al. (2015). Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane. CELL CALCIUM, 57(2), 57-68 [10.1016/j.ceca.2014.12.003].
Citazione: | RIGAMONTI, M., GROPPI, S., BELOTTI, F., AMBROSINI, R., FILIPPI, G., MARTEGANI, E., et al. (2015). Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane. CELL CALCIUM, 57(2), 57-68 [10.1016/j.ceca.2014.12.003]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane | |
Autori: | RIGAMONTI, M; GROPPI, S; BELOTTI, F; AMBROSINI, R; FILIPPI, G; MARTEGANI, E; TISI, R | |
Autori: | ||
Data di pubblicazione: | 2015 | |
Lingua: | English | |
Rivista: | CELL CALCIUM | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.ceca.2014.12.003 | |
Appare nelle tipologie: | 01 - Articolo su rivista |
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