A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.

Tisi, R., Martegani, E., Brandão, R. (2015). Total cellular Ca2+ measurements in yeast using flame photometry. COLD SPRING HARBOR PROTOCOLS, 2015(2), 214-216 [10.1101/pdb.prot076869].

Total cellular Ca2+ measurements in yeast using flame photometry

TISI, RENATA ANITA
Primo
;
MARTEGANI, ENZO
Secondo
;
2015

Abstract

A photoelectric flame photometer is a device used in inorganic chemical analysis for determining the concentrations of certain metals in solution. It does this by measuring the intensity of the light emitted by the metal when the solution is sprayed under controlled conditions into a nonluminous flame. This protocol describes how to measure total cellular calcium (maximal emission at 622 nm, orange flame) in yeast using this technique.
Articolo in rivista - Articolo scientifico
calcium; budding yeast; Saccharomyces cerevisiae
English
2015
2015
2
214
216
none
Tisi, R., Martegani, E., Brandão, R. (2015). Total cellular Ca2+ measurements in yeast using flame photometry. COLD SPRING HARBOR PROTOCOLS, 2015(2), 214-216 [10.1101/pdb.prot076869].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/77637
Citazioni
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
Social impact