Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells. Since when EB is bound to nucleic acids its fluorescence is intensified; a higher EB fluorescence intensity could reflect a higher DNA accessibility to EB, suggesting a higher mtDNA replication activity. To prove this hypothesis, in the present work we studied, by LSCFM, the EB fluorescence in mitochondria nucleoids of living neuroblastoma cells, a model system in which differentiation affects the level of mtDNA replication. A drastic decrease of fluorescence was observed after differentiation. To correlate EB fluorescence intensity to the mtDNA replication state, we evaluated the mtDNA nascent strands content by ligation-mediated real-time PCR, and we found a halved amount of replicating mtDNA molecules in differentiating cells. A similar result was obtained by BrdU incorporation. These results indicate that the low EB fluorescence of nucleoids in differentiated cells is correlated to a low content of replicating mtDNA, suggesting that EB may be used as a marker of mtDNA replication in living cells. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).

Villa, A., Fusi, P., Pastori, V., Amicarelli, G., Pozzi, C., Adlerstein, D., et al. (2012). Ethidium bromide as a marker of mtDNA replication in living cells. JOURNAL OF BIOMEDICAL OPTICS, 17(4), 046001 [10.1117/1.JBO.17.4.046001].

Ethidium bromide as a marker of mtDNA replication in living cells

VILLA, ANNA MARIA
;
FUSI, PAOLA ALESSANDRA
Secondo
;
PASTORI, VALENTINA;DOGLIA, SILVIA MARIA
Ultimo
2012

Abstract

Mitochondrial DNA (mtDNA) in tumor cells was found to play an important role in maintaining the malignant phenotype. Using laser scanning confocal fluorescence microscopy (LSCFM) in a recent work, we reported a variable fluorescence intensity of ethidium bromide (EB) in mitochondria nucleoids of living carcinoma cells. Since when EB is bound to nucleic acids its fluorescence is intensified; a higher EB fluorescence intensity could reflect a higher DNA accessibility to EB, suggesting a higher mtDNA replication activity. To prove this hypothesis, in the present work we studied, by LSCFM, the EB fluorescence in mitochondria nucleoids of living neuroblastoma cells, a model system in which differentiation affects the level of mtDNA replication. A drastic decrease of fluorescence was observed after differentiation. To correlate EB fluorescence intensity to the mtDNA replication state, we evaluated the mtDNA nascent strands content by ligation-mediated real-time PCR, and we found a halved amount of replicating mtDNA molecules in differentiating cells. A similar result was obtained by BrdU incorporation. These results indicate that the low EB fluorescence of nucleoids in differentiated cells is correlated to a low content of replicating mtDNA, suggesting that EB may be used as a marker of mtDNA replication in living cells. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).
Articolo in rivista - Articolo scientifico
BrdU; Confocal fluorescence microscopy; Ethidium bromide; Human neuroblastoma cells; Ligation-mediated PCR; Mitochondria; MtDNA nucleoids mtDNA replication; Bromodeoxyuridine; Cell Line, Tumor; DNA, Mitochondrial; Ethidium; Fluorescent Dyes; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Microscopy, Fluorescence; Mitochondria; DNA Replication; Biomedical Engineering; Biomaterials; Electronic, Optical and Magnetic Materials; Atomic and Molecular Physics, and Optics
English
2012
17
4
046001
046001
none
Villa, A., Fusi, P., Pastori, V., Amicarelli, G., Pozzi, C., Adlerstein, D., et al. (2012). Ethidium bromide as a marker of mtDNA replication in living cells. JOURNAL OF BIOMEDICAL OPTICS, 17(4), 046001 [10.1117/1.JBO.17.4.046001].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/75256
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