Because progressive fibrosis is a histological hallmark of the aging kidney, we sought to characterize the course of some fibrosis-related genes [pro-alpha2(I)collagen (COL-I), pro-alpha1(III)collagen (COL-III), and transforming growth Factors beta1 and beta3 (TGF-beta1 and TGF-beta3)] of interstitial collagen accumulation [COL-I and COL-III proteins, hydroxyproline (PRO-OH), histology] and its degradation (matrix metalloproteinase MMP-1 and -2) during maturation and early aging in rats. During the lifespan considered we observed no changes in the mRNA, except that COL-I mRNA tended to be up-regulated from 2 to 19 months of age. However, progressive fibrosis was histologically detectable, with COL-I accumulation (p <.05 and p <.01 in 12-month- and 19-month-old rats vs the youngest), and confirmed by the PRO-OH tissue levels (p =.0001); COL-III seemed to be less involved. The MMP-1 protein level decreased significantly in the cortex of 12-month- and 19-month-old rats (p <.05), whereas MMP-2 protein level and activity remained essentially unchanged. These results show that, during aging of the kidney, (i) renal cortex fibrosis is explained by COL-I accumulation as a consequence of an altered balance between its synthesis and degradation, and (ii) the expression of the pleiotropic factor TGF-<beta> in the renal cortex is not modified.

Gagliano, N., Arosio, B., Santambrogio, D., Balestrieri, M., Padoani, G., Tagliabue, J., et al. (2000). Age-dependent expression of fibrosis-related genes and collagen deposition in rat kidney cortex. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 55(8), B365-B372.

Age-dependent expression of fibrosis-related genes and collagen deposition in rat kidney cortex

ANNONI, GIORGIO
2000-08

Abstract

Because progressive fibrosis is a histological hallmark of the aging kidney, we sought to characterize the course of some fibrosis-related genes [pro-alpha2(I)collagen (COL-I), pro-alpha1(III)collagen (COL-III), and transforming growth Factors beta1 and beta3 (TGF-beta1 and TGF-beta3)] of interstitial collagen accumulation [COL-I and COL-III proteins, hydroxyproline (PRO-OH), histology] and its degradation (matrix metalloproteinase MMP-1 and -2) during maturation and early aging in rats. During the lifespan considered we observed no changes in the mRNA, except that COL-I mRNA tended to be up-regulated from 2 to 19 months of age. However, progressive fibrosis was histologically detectable, with COL-I accumulation (p <.05 and p <.01 in 12-month- and 19-month-old rats vs the youngest), and confirmed by the PRO-OH tissue levels (p =.0001); COL-III seemed to be less involved. The MMP-1 protein level decreased significantly in the cortex of 12-month- and 19-month-old rats (p <.05), whereas MMP-2 protein level and activity remained essentially unchanged. These results show that, during aging of the kidney, (i) renal cortex fibrosis is explained by COL-I accumulation as a consequence of an altered balance between its synthesis and degradation, and (ii) the expression of the pleiotropic factor TGF- in the renal cortex is not modified.
Articolo in rivista - Articolo scientifico
extracellular-matrix, nephropathy, TGF-beta1
English
Gagliano, N., Arosio, B., Santambrogio, D., Balestrieri, M., Padoani, G., Tagliabue, J., et al. (2000). Age-dependent expression of fibrosis-related genes and collagen deposition in rat kidney cortex. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 55(8), B365-B372.
Gagliano, N; Arosio, B; Santambrogio, D; Balestrieri, M; Padoani, G; Tagliabue, J; Masson, S; Vergani, C; Annoni, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/750
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