The stromal cell-derived factor (SDF)-1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF-1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real-time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF-1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF-1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF-1 release is decreased. SDF-1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F-benzoyl-TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF-1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors. © 2010 Wiley Periodicals, Inc.

Melchionna, R., Di Carlo, A., De Mori, R., Cappuzzello, C., Barberi, L., Musarò, A., et al. (2010). Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors. MUSCLE & NERVE, 41(6), 828-835 [10.1002/mus.21611].

Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors

CAPPUZZELLO, CLAUDIA;
2010

Abstract

The stromal cell-derived factor (SDF)-1/CXC receptor 4 (CXCR4) axis has been shown to play a role in skeletal muscle development, but its contribution to postnatal myogenesis and the role of the alternate SDF-1 receptor, CXC receptor 7 (CXCR7), are poorly characterized. Western blot analysis and real-time polymerase chain reaction (PCR) were performed to evaluate in vitro the effect of SDF-1 and CXCR4 and CXCR7 inhibition on myogenic differentiation. Proliferating myoblasts express CXCR4, CXCR7, and SDF-1; during myogenic differentiation, CXCR4 and CXCR7 levels are downregulated, and SDF-1 release is decreased. SDF-1 anticipates myosin heavy chain accumulation and myotube formation in both C2C12 myoblasts and satellite cells. Interestingly, inhibition of CXCR4 and CXCR7 signaling, either by drugs or RNA interfererence, blocks myogenic differentiation. Further, the CXCR4 antagonist, 4F-benzoyl-TN14003, inhibits myoblast cell cycle withdrawal and decreases the retinoblastoma gene (pRb) product accumulation in its hypophosphorylated form. Our experiments demonstrate that SDF-1 regulates myogenic differentiation via both CXCR4 and CXCR7 chemokine receptors. © 2010 Wiley Periodicals, Inc.
Articolo in rivista - Articolo scientifico
Chemokine; Myogenesis; Regeneration; Satellite cells; SDF-1; Animals; Cell Cycle; Cell Differentiation; Chemokine CXCL12; Flow Cytometry; Mice; Myoblasts; Polymerase Chain Reaction; RNA, Messenger; RNA, Small Interfering; Receptors, CXCR; Receptors, CXCR4; Neurology (clinical); Cellular and Molecular Neuroscience; Physiology (medical); Physiology;
English
2010
41
6
828
835
none
Melchionna, R., Di Carlo, A., De Mori, R., Cappuzzello, C., Barberi, L., Musarò, A., et al. (2010). Induction of myogenic differentiation by SDF-1 via CXCR4 and CXCR7 receptors. MUSCLE & NERVE, 41(6), 828-835 [10.1002/mus.21611].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/70268
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