Intracranial collaterals are dynamically recruited after arterial occlusion and are emerging as a strong determinant of tissue outcome in both human and experimental ischemic stroke. The relationship between collateral flow and ischemic penumbra remains largely unexplored in pre-clinical studies. The aim of the present study was to investigate the pattern of collateral flow with regard to penumbral tissue after transient middle cerebral artery (MCA) occlusion in rats. MCA was transiently occluded (90. min) by intraluminal filament in adult male Wistar rats (n. =. 25). Intracranial collateral flow was studied in terms of perfusion deficit and biosignal fluctuation analyses using multi-site laser Doppler monitoring. Molecular penumbra was defined by topographical mapping and quantitative signal analysis of Heat Shock Protein 70. kDa (HSP70) immunohistochemistry. Functional deficit and infarct volume were assessed 24. h after ischemia induction. The results show that functional performance of intracranial collaterals during MCA occlusion inversely correlated with HSP70 immunoreactive areas in both the cortex and the striatum, as well as with infarct size and functional deficit. Intracranial collateral flow was associated with reduced areas of both molecular penumbra and ischemic core and increased areas of intact tissue in rats subjected to MCA occlusion followed by reperfusion. Our findings prompt the development of collateral therapeutics to provide tissue-saving strategies in the hyper-acute phase of ischemic stroke prior to recanalization therapy.

Beretta, S., Cuccione, E., Versace, A., Carone, D., Riva, M., Padovano, G., et al. (2015). Cerebral collateral flow defines topography and evolution of molecular penumbra in experimental ischemic stroke. NEUROBIOLOGY OF DISEASE, 74, 305-313 [10.1016/j.nbd.2014.11.019].

Cerebral collateral flow defines topography and evolution of molecular penumbra in experimental ischemic stroke

Beretta, S;CUCCIONE, ELISA;CARONE, DAVIDE;RIVA, MATTEO;PADOVANO, GIADA;MONZA, LAURA;PRESOTTO, LUCA;GIUSSANI, CARLO GIORGIO;SGANZERLA, ERIK PIETRO;FERRARESE, CARLO
2015

Abstract

Intracranial collaterals are dynamically recruited after arterial occlusion and are emerging as a strong determinant of tissue outcome in both human and experimental ischemic stroke. The relationship between collateral flow and ischemic penumbra remains largely unexplored in pre-clinical studies. The aim of the present study was to investigate the pattern of collateral flow with regard to penumbral tissue after transient middle cerebral artery (MCA) occlusion in rats. MCA was transiently occluded (90. min) by intraluminal filament in adult male Wistar rats (n. =. 25). Intracranial collateral flow was studied in terms of perfusion deficit and biosignal fluctuation analyses using multi-site laser Doppler monitoring. Molecular penumbra was defined by topographical mapping and quantitative signal analysis of Heat Shock Protein 70. kDa (HSP70) immunohistochemistry. Functional deficit and infarct volume were assessed 24. h after ischemia induction. The results show that functional performance of intracranial collaterals during MCA occlusion inversely correlated with HSP70 immunoreactive areas in both the cortex and the striatum, as well as with infarct size and functional deficit. Intracranial collateral flow was associated with reduced areas of both molecular penumbra and ischemic core and increased areas of intact tissue in rats subjected to MCA occlusion followed by reperfusion. Our findings prompt the development of collateral therapeutics to provide tissue-saving strategies in the hyper-acute phase of ischemic stroke prior to recanalization therapy.
Articolo in rivista - Articolo scientifico
Cerebral collaterals; Hemodynamic changes; Neuroprotection; Penumbra; Stroke;
Cerebral collaterals; Hemodynamic changes; Neuroprotection; Penumbra; Stroke; Neurology
English
2015
74
305
313
reserved
Beretta, S., Cuccione, E., Versace, A., Carone, D., Riva, M., Padovano, G., et al. (2015). Cerebral collateral flow defines topography and evolution of molecular penumbra in experimental ischemic stroke. NEUROBIOLOGY OF DISEASE, 74, 305-313 [10.1016/j.nbd.2014.11.019].
File in questo prodotto:
File Dimensione Formato  
Cerebral collateral flow.pdf

Solo gestori archivio

Dimensione 1.68 MB
Formato Adobe PDF
1.68 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/66516
Citazioni
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 19
Social impact