A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O 6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence. © 2013 Copyright SPIE.

Mazzucchelli, S., Colombo, M., Galbiati, E., Corsi, F., Montenegro, J., Parak, W., et al. (2013). O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles. PROGRESS IN BIOMEDICAL OPTICS AND IMAGING, 8595, 859502 [10.1117/12.2001648].

O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles

MAZZUCCHELLI, SERENA
;
COLOMBO, MIRIAM
Secondo
;
GALBIATI, ELISABETTA;PROSPERI, DAVIDE
Ultimo
2013

Abstract

A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O 6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence. © 2013 Copyright SPIE.
Articolo in rivista - Articolo scientifico
bioconjugation; breast cancer; capture module; fusion protein; magnetic nanoparticles; scFv; targeting module; tumor targeting; Atomic and Molecular Physics, and Optics; Electronic, Optical and Magnetic Materials; Biomaterials; Radiology, Nuclear Medicine and Imaging
English
2013
8595
859502
859502
none
Mazzucchelli, S., Colombo, M., Galbiati, E., Corsi, F., Montenegro, J., Parak, W., et al. (2013). O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles. PROGRESS IN BIOMEDICAL OPTICS AND IMAGING, 8595, 859502 [10.1117/12.2001648].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/64502
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