Dendritic cells (DCs) are strong activators of primary T cell responses. Their priming ability is acquired upon encounter with maturation stimuli. To identify the genes that are differentially expressed upon maturation induced by exposure to Gram-negative bacteria, a kinetic study of DC gene expression was done with microarrays representing 11,000 genes and ESTs (expressed sequence tags). Approximately 3000 differentially expressed transcripts were identified. We found that functional interleukin 2 (IL-2) mRNA, which gave rise to IL-2 production, was transiently up. regulated at early time-points after bacterial encounter. In contrast, macrophages did not produce IL-2 upon bacterial stimulation. Thus, IL-2 is an additional key cytokine that confers unique T cell stimulatory capacity to DCs.

Granucci, F., Vizzardelli, C., Pavelka, N., Feau, S., Persico, M., Virzi, E., et al. (2001). Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. NATURE IMMUNOLOGY, 2(9), 882-888 [10.1038/ni0901-882].

Inducible IL-2 production by dendritic cells revealed by global gene expression analysis

GRANUCCI, FRANCESCA;MORO, GIORGIO;CASTAGNOLI, PAOLA
2001

Abstract

Dendritic cells (DCs) are strong activators of primary T cell responses. Their priming ability is acquired upon encounter with maturation stimuli. To identify the genes that are differentially expressed upon maturation induced by exposure to Gram-negative bacteria, a kinetic study of DC gene expression was done with microarrays representing 11,000 genes and ESTs (expressed sequence tags). Approximately 3000 differentially expressed transcripts were identified. We found that functional interleukin 2 (IL-2) mRNA, which gave rise to IL-2 production, was transiently up. regulated at early time-points after bacterial encounter. In contrast, macrophages did not produce IL-2 upon bacterial stimulation. Thus, IL-2 is an additional key cytokine that confers unique T cell stimulatory capacity to DCs.
Articolo in rivista - Articolo scientifico
Dendritic cells, gene expression
English
882
888
7
Granucci, F., Vizzardelli, C., Pavelka, N., Feau, S., Persico, M., Virzi, E., et al. (2001). Inducible IL-2 production by dendritic cells revealed by global gene expression analysis. NATURE IMMUNOLOGY, 2(9), 882-888 [10.1038/ni0901-882].
Granucci, F; Vizzardelli, C; Pavelka, N; Feau, S; Persico, M; Virzi, E; Rescigno, M; Moro, G; Castagnoli, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/63677
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