This chapter aims to provide a practical guide to performing comprehensive spatial lipidomics of formalin-fixed paraffin-embedded (FFPE) tissue guided by Matrix Assisted Laser Desorption/Ionisation-mass spectrometry-imaging (MALDI-MSI), presenting an overview of the key methodological aspects as well as the type of data that can be obtained when using this approach. Moreover, it also aims to highlight the more extensive and reliable lipid identifications that can be obtained when an additional trapped ion mobility spectrometry (TIMS) dimension is employed.
Denti, V., Piazza, M., Smith, A., Paglia, G. (2023). Comprehensive Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Tissue Guided by Mass Spectrometry-Imaging. In J. Ivanisevic, M. Giera (a cura di), A Practical Guide to Metabolomics Applications in Health and Disease From Samples to Insights into Metabolism (pp. 373-388). Springer Nature [10.1007/978-3-031-44256-8_14].
Comprehensive Spatial Lipidomics of Formalin-Fixed Paraffin-Embedded Tissue Guided by Mass Spectrometry-Imaging
Denti V.;Smith A.;Paglia G.
2023
Abstract
This chapter aims to provide a practical guide to performing comprehensive spatial lipidomics of formalin-fixed paraffin-embedded (FFPE) tissue guided by Matrix Assisted Laser Desorption/Ionisation-mass spectrometry-imaging (MALDI-MSI), presenting an overview of the key methodological aspects as well as the type of data that can be obtained when using this approach. Moreover, it also aims to highlight the more extensive and reliable lipid identifications that can be obtained when an additional trapped ion mobility spectrometry (TIMS) dimension is employed.
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