Intrinsically disordered proteins (IDPs) carry out important biological functions and offer an instructive model system for folding and binding studies. However, their structural characterization in the absence of interactors is hindered by their highly dynamic conformation. The cyclin-dependent-kinase inhibitor (Cki) Sic1 from Saccharomyces cerevisiae is a key regulator of the yeast cell cycle, which controls entrance into S phase and coordination between cell growth and proliferation. Its last 70 out of 284 residues display functional and structural homology to the inhibitory domain of mammalian p21 and p27. Sic1 has escaped systematic structural characterization until now. Here, complementary biophysical methods are applied to the study of conformational properties of pure Sic1 in solution. Based on sequence analysis, gel filtration, circular dichroism (CD), electrospray-ionization mass spectrometry (ESI-MS), and limited proteolysis, it can be concluded that the whole molecule exists in a highly disordered state and can, therefore, be classified as an IDP. However, the results of these experiments indicate, at the same time, that the protein displays some content in secondary and tertiary structure, having properties similar to those of molten globules or premolten globules. Proteolysis-hypersensitive sites cluster at the N-terminus and in the middle of the molecule, whereas the most structured region resides at the C-terminus, including part of the inhibitory domain and the casein-kinase-2 (CK2) phosphorylation target S201. The mutations S201A and S201E, which are known to affect Sic1 function, do not have significant effects on the conformational properties of the pure protein. Proteins 2009;76:731-746. (c) 2009 Wiley-Liss, Inc.

Brocca, S., Samalikova, M., Uversky, V., Lotti, M., Vanoni, M., Alberghina, L., et al. (2009). Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1. PROTEINS, 76(3), 731-746 [10.1002/prot.22385].

Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1

BROCCA, STEFANIA;SAMALIKOVA, MARIA;LOTTI, MARINA;VANONI, MARCO ERCOLE;ALBERGHINA, LILIA;GRANDORI, RITA
2009

Abstract

Intrinsically disordered proteins (IDPs) carry out important biological functions and offer an instructive model system for folding and binding studies. However, their structural characterization in the absence of interactors is hindered by their highly dynamic conformation. The cyclin-dependent-kinase inhibitor (Cki) Sic1 from Saccharomyces cerevisiae is a key regulator of the yeast cell cycle, which controls entrance into S phase and coordination between cell growth and proliferation. Its last 70 out of 284 residues display functional and structural homology to the inhibitory domain of mammalian p21 and p27. Sic1 has escaped systematic structural characterization until now. Here, complementary biophysical methods are applied to the study of conformational properties of pure Sic1 in solution. Based on sequence analysis, gel filtration, circular dichroism (CD), electrospray-ionization mass spectrometry (ESI-MS), and limited proteolysis, it can be concluded that the whole molecule exists in a highly disordered state and can, therefore, be classified as an IDP. However, the results of these experiments indicate, at the same time, that the protein displays some content in secondary and tertiary structure, having properties similar to those of molten globules or premolten globules. Proteolysis-hypersensitive sites cluster at the N-terminus and in the middle of the molecule, whereas the most structured region resides at the C-terminus, including part of the inhibitory domain and the casein-kinase-2 (CK2) phosphorylation target S201. The mutations S201A and S201E, which are known to affect Sic1 function, do not have significant effects on the conformational properties of the pure protein. Proteins 2009;76:731-746. (c) 2009 Wiley-Liss, Inc.
Articolo in rivista - Articolo scientifico
disorder prediction, protein-protein interactions, protein folding, molten globule, limited proteolysis, mass spectrometry, circular dichroism, protein phosphorylation, cell cycle, Saccharomyces cerevisiae
English
Brocca, S., Samalikova, M., Uversky, V., Lotti, M., Vanoni, M., Alberghina, L., et al. (2009). Order propensity of an intrinsically disordered protein, the cyclin-dependent-kinase inhibitor Sic1. PROTEINS, 76(3), 731-746 [10.1002/prot.22385].
Brocca, S; Samalikova, M; Uversky, V; Lotti, M; Vanoni, M; Alberghina, L; Grandori, R
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/6049
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