Since the early 1990s, electrospray-ionization mass spectrometry (ESI-MS) has encountered growing interest as a complementary tool to established biochemical and biophysical methods for investigating protein structure and conformation. Nowadays, applications of ESI-MS to protein investigation span from the area of analytical biochemistry to that of structural biology. This review focuses on applications of this technique to the analysis of protein conformational properties and molecular interactions, underscoring their possible relevance for molecular biotechnology, although representing a still very young field. An introductive section presents the major issues related to theoretical and technical aspects of ESI-MS under non-denaturing conditions. Examples from our work and from the literature illustrate which kind of information can be obtained concerning key issues in biotechnology such as stability and aggregation of proteins under both near-native and challenging conditions, and interactions with other proteins, ligands and cofactors

Grandori, R., Santambrogio, C., Brocca, S., Invernizzi, G., Lotti, M. (2009). Electrospray-ionization mass spectrometry as a tool for fast screening of protein structural properties. BIOTECHNOLOGY JOURNAL, 4(1), 73-87 [10.1002/biot.200800250].

Electrospray-ionization mass spectrometry as a tool for fast screening of protein structural properties

GRANDORI, RITA;SANTAMBROGIO, CARLO;BROCCA, STEFANIA;INVERNIZZI, GAETANO;LOTTI, MARINA
2009

Abstract

Since the early 1990s, electrospray-ionization mass spectrometry (ESI-MS) has encountered growing interest as a complementary tool to established biochemical and biophysical methods for investigating protein structure and conformation. Nowadays, applications of ESI-MS to protein investigation span from the area of analytical biochemistry to that of structural biology. This review focuses on applications of this technique to the analysis of protein conformational properties and molecular interactions, underscoring their possible relevance for molecular biotechnology, although representing a still very young field. An introductive section presents the major issues related to theoretical and technical aspects of ESI-MS under non-denaturing conditions. Examples from our work and from the literature illustrate which kind of information can be obtained concerning key issues in biotechnology such as stability and aggregation of proteins under both near-native and challenging conditions, and interactions with other proteins, ligands and cofactors
Articolo in rivista - Articolo scientifico
Electrospray-ionization mass spectrometry, Protein conformation, Protein-ligand interactions, Protein-protein interactions
English
73
87
Grandori, R., Santambrogio, C., Brocca, S., Invernizzi, G., Lotti, M. (2009). Electrospray-ionization mass spectrometry as a tool for fast screening of protein structural properties. BIOTECHNOLOGY JOURNAL, 4(1), 73-87 [10.1002/biot.200800250].
Grandori, R; Santambrogio, C; Brocca, S; Invernizzi, G; Lotti, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/6047
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