Allele and genotype frquencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0.65 to 0.78. Two point and three point linkage analysis among these markers and HLA-A serological types - available for all the studied samples - allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage anlaysis of genetic disorders mapping on this region of 6 p.
Camaschella, C., Roetto, A., De Sandre, G., Piperno, A., Totaro, A., Dianzani, I., et al. (1993). Construction of a genetic map telomeric to HAL-A by microsatellite analysis. MOLECULAR AND CELLULAR PROBES, 7(5), 411-414 [10.1006/mcpr.1993.1060].
Construction of a genetic map telomeric to HAL-A by microsatellite analysis
PIPERNO, ALBERTO;
1993
Abstract
Allele and genotype frquencies of five microsatellites on the short arm of chromosome 6 have been determined in 158 subjects from 14 large Italian families, using polymerase chain reaction amplification and a non-radioactive method of detection. The markers studied were: D6S89, D6S109, D6S105, pLR12 and HLA-F; their heterozygosity ranged from 0.65 to 0.78. Two point and three point linkage analysis among these markers and HLA-A serological types - available for all the studied samples - allowed construction of a partial genetic map telomeric to HLA-A. Our results may be useful for linkage anlaysis of genetic disorders mapping on this region of 6 p.
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