Monoclonal antibodies (mAbs) with a high sensitivity and specificity for small cell lung cancer (SCLC) may be of potential diagnostic and therapeutic use. We selected five different mAbs generated against SCLC cell lines and tested them on paraffin-embedded SCLC and non SCLC (NSCLC) clinical samples in a retrospective study using immunohistochemical techniques. The results showed that the contemporary use of mAbs that react with different antigens allowed all the examined SCLC to be detected indicating that this panel of mAbs was extremely sensitive. Analysis of the reactivity of these mAbs with NSCLC showed that 4 of the 5 antibodies reacted also with a few NSCLC. Using higher dilutions of these mAbs their specificity improved substantially. The 5 different mAbs presented marked heterogeneity of antigenic expression within and between SCLC. This panel of antibodies may be very useful in the diagnosis of SCLC whereas their application, in vivo, in therapeutic trials, is limited by the heterogeneity of antigenic expression.

Fargion, S., Boeri, R., Cislaghi, V., Francanzani, A., Piperno, A., Schmid, C., et al. (1988). Immunohistochemical evaluation of a panel of monoclonal antibodies for the diagnosis of small cell lung cancer. TUMORI, 74(6), 713-718.

Immunohistochemical evaluation of a panel of monoclonal antibodies for the diagnosis of small cell lung cancer

PIPERNO, ALBERTO;
1988

Abstract

Monoclonal antibodies (mAbs) with a high sensitivity and specificity for small cell lung cancer (SCLC) may be of potential diagnostic and therapeutic use. We selected five different mAbs generated against SCLC cell lines and tested them on paraffin-embedded SCLC and non SCLC (NSCLC) clinical samples in a retrospective study using immunohistochemical techniques. The results showed that the contemporary use of mAbs that react with different antigens allowed all the examined SCLC to be detected indicating that this panel of mAbs was extremely sensitive. Analysis of the reactivity of these mAbs with NSCLC showed that 4 of the 5 antibodies reacted also with a few NSCLC. Using higher dilutions of these mAbs their specificity improved substantially. The 5 different mAbs presented marked heterogeneity of antigenic expression within and between SCLC. This panel of antibodies may be very useful in the diagnosis of SCLC whereas their application, in vivo, in therapeutic trials, is limited by the heterogeneity of antigenic expression.
Articolo in rivista - Articolo scientifico
Antibodies, Monoclonal; Antigens, Neoplasm; Carcinoma, Non-Small-Cell Lung; Carcinoma, Small Cell; Humans; Immunohistochemistry; Lung Neoplasms
English
713
718
6
Fargion, S., Boeri, R., Cislaghi, V., Francanzani, A., Piperno, A., Schmid, C., et al. (1988). Immunohistochemical evaluation of a panel of monoclonal antibodies for the diagnosis of small cell lung cancer. TUMORI, 74(6), 713-718.
Fargion, S; Boeri, R; Cislaghi, V; Francanzani, A; Piperno, A; Schmid, C; Fiorelli, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/58161
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