Purified recombinant human ferritin composed solely of H subunit was radiolabeled and incubated with proerythroleukemic K562 human cells. A specific binding was detected, and it could be displaced only by ferritins, natural or recombinant, containing large proportion of the H subunit. The specific ferritin H-chain binding was saturable, and cells showed 17,000 to 23,000 binding sites per cell. The affinity constant measured at 37 degrees C was of 3 x 10(8) M-1. Treatment with pronase eliminated the specific binding. The binding sites were expressed in a high number during the cellular exponential phase of growth and progressively decreased to disappear when cells reached the plateau phase. Treatment of the cells with desferrioxamine increased recombinant H-ferritin binding, while iron had little effect. K562 cells induced to differentiate by hemin failed to bind ferritin H. Ferritin H-chain binding capacity is present on various cell lines such as HL60, lung cancer, and hepatoma cells. Analysis of the binding sites by western blotting showed a peptide with apparent mol wt of about 100 kd.

Fargion, S., Arosio, P., Fracanzani, A., Cislaghi, V., Levi, S., Cozzi, A., et al. (1988). Characteristics and expression of binding sites specific for ferritin H-chain on human cell lines. BLOOD, 71(3), 753-757.

Characteristics and expression of binding sites specific for ferritin H-chain on human cell lines

PIPERNO, ALBERTO
Penultimo
;
1988

Abstract

Purified recombinant human ferritin composed solely of H subunit was radiolabeled and incubated with proerythroleukemic K562 human cells. A specific binding was detected, and it could be displaced only by ferritins, natural or recombinant, containing large proportion of the H subunit. The specific ferritin H-chain binding was saturable, and cells showed 17,000 to 23,000 binding sites per cell. The affinity constant measured at 37 degrees C was of 3 x 10(8) M-1. Treatment with pronase eliminated the specific binding. The binding sites were expressed in a high number during the cellular exponential phase of growth and progressively decreased to disappear when cells reached the plateau phase. Treatment of the cells with desferrioxamine increased recombinant H-ferritin binding, while iron had little effect. K562 cells induced to differentiate by hemin failed to bind ferritin H. Ferritin H-chain binding capacity is present on various cell lines such as HL60, lung cancer, and hepatoma cells. Analysis of the binding sites by western blotting showed a peptide with apparent mol wt of about 100 kd.
Articolo in rivista - Articolo scientifico
Binding Sites; Cell Line; Chemical Phenomena; Chemistry; Ferritins; Recombinant Proteins; Tumor Cells, Cultured
English
753
757
5
Fargion, S., Arosio, P., Fracanzani, A., Cislaghi, V., Levi, S., Cozzi, A., et al. (1988). Characteristics and expression of binding sites specific for ferritin H-chain on human cell lines. BLOOD, 71(3), 753-757.
Fargion, S; Arosio, P; Fracanzani, A; Cislaghi, V; Levi, S; Cozzi, A; Piperno, A; Fiorelli, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/58157
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