G-protein-coupled receptors (GPCRs) are integral membrane proteins, hence it is not surprising that a number of their structural and functional features are modulated by both proteins and lipids. The impact of interacting proteins and lipids on the assembly and signalling of GPCRs has been extensively investigated over the last 20-30 years, and a further impetus has been given by the proposal that GPCRs and/or their immediate signalling partners (G proteins) can partition within plasma membrane domains, termed rafts and caveolae, enriched in glycosphingolipids and cholesterol. The high content of these specific lipids, in particular of cholesterol, in the vicinity of GPCR transmembranes can affect GPCR structure and/or function. In addition, most GPCRs are post-translationally modified with one or more palmitic acid(s), a 16-carbon saturated fatty acid, covalently bound to cysteine(s) localised in the carboxyl-terminal cytoplasmic tail. The insertion of palmitate into the cytoplasmic leaflet of the plasma membrane can create a fourth loop, thus profoundly affecting GPCR structure and hence the interactions with intracellular partner proteins. This review briefly highlights how lipids of the membrane and the receptor themselves can influence GPCR organisation and functioning. © 2009 Society for Endocrinology.

Chini, B., Parenti, M. (2009). G-protein-coupled receptors, cholesterol and palmitoylation: Facts about fats. JOURNAL OF MOLECULAR ENDOCRINOLOGY, 42(5), 371-379 [10.1677/JME-08-0114].

G-protein-coupled receptors, cholesterol and palmitoylation: Facts about fats

PARENTI, MARCO DOMENICO
2009

Abstract

G-protein-coupled receptors (GPCRs) are integral membrane proteins, hence it is not surprising that a number of their structural and functional features are modulated by both proteins and lipids. The impact of interacting proteins and lipids on the assembly and signalling of GPCRs has been extensively investigated over the last 20-30 years, and a further impetus has been given by the proposal that GPCRs and/or their immediate signalling partners (G proteins) can partition within plasma membrane domains, termed rafts and caveolae, enriched in glycosphingolipids and cholesterol. The high content of these specific lipids, in particular of cholesterol, in the vicinity of GPCR transmembranes can affect GPCR structure and/or function. In addition, most GPCRs are post-translationally modified with one or more palmitic acid(s), a 16-carbon saturated fatty acid, covalently bound to cysteine(s) localised in the carboxyl-terminal cytoplasmic tail. The insertion of palmitate into the cytoplasmic leaflet of the plasma membrane can create a fourth loop, thus profoundly affecting GPCR structure and hence the interactions with intracellular partner proteins. This review briefly highlights how lipids of the membrane and the receptor themselves can influence GPCR organisation and functioning. © 2009 Society for Endocrinology.
Articolo in rivista - Articolo scientifico
palmytoilation
English
2009
42
5
371
379
none
Chini, B., Parenti, M. (2009). G-protein-coupled receptors, cholesterol and palmitoylation: Facts about fats. JOURNAL OF MOLECULAR ENDOCRINOLOGY, 42(5), 371-379 [10.1677/JME-08-0114].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/5457
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