During CNS development neurons undergo directional migration to achieve their adult localizations. To study neuronal migration, we used a model cell line of immortalized murine neurons (gonadotropin-releasing hormone expressing neurons; GN11), enriched with caveolins and caveolae invaginations that show in vitro chemotaxis upon serum exposure. Cholesterol depletion with methyl-beta-cyclodextrin induced the loss of caveolae and the inhibition of chemotaxis, thus suggesting that GN11 migration depends upon the structural integrity of caveolae. Polarization of proteins and organelles is a hallmark of cell migration. Accordingly, GN11 cells transmigrating through filter pores exhibited a polarized distribution of caveolin-1 isoform (cav-1) in the leading processes. In contrast, during two-dimensional migration cav-1 and caveolae polarized at the trailing edge. As caveolae are enriched with signaling molecules, we suggest that asymmetrical localization of caveolae may spatially orient GN11 neurons to incoming migratory signals thereby transducing them into directional migration.

Lentini, D., Guzzi, F., Pimpinelli, F., Zaninetti, R., Cassetti, A., Coco, S., et al. (2008). Polarization of caveolins and caveolae during migration of immortalized neurons. JOURNAL OF NEUROCHEMISTRY, 104(2), 514-523 [10.1111/j.1471-4159.2007.05005.x].

Polarization of caveolins and caveolae during migration of immortalized neurons

LENTINI, DANIELA;GUZZI, FRANCESCA;COCO, SILVIA;PARENTI, MARCO DOMENICO
2008

Abstract

During CNS development neurons undergo directional migration to achieve their adult localizations. To study neuronal migration, we used a model cell line of immortalized murine neurons (gonadotropin-releasing hormone expressing neurons; GN11), enriched with caveolins and caveolae invaginations that show in vitro chemotaxis upon serum exposure. Cholesterol depletion with methyl-beta-cyclodextrin induced the loss of caveolae and the inhibition of chemotaxis, thus suggesting that GN11 migration depends upon the structural integrity of caveolae. Polarization of proteins and organelles is a hallmark of cell migration. Accordingly, GN11 cells transmigrating through filter pores exhibited a polarized distribution of caveolin-1 isoform (cav-1) in the leading processes. In contrast, during two-dimensional migration cav-1 and caveolae polarized at the trailing edge. As caveolae are enriched with signaling molecules, we suggest that asymmetrical localization of caveolae may spatially orient GN11 neurons to incoming migratory signals thereby transducing them into directional migration.
Articolo in rivista - Articolo scientifico
caveolae; caveolin; GN11 cells; neuronal migration; cholesterol; beta-cyclodextrin
English
gen-2008
104
2
514
523
none
Lentini, D., Guzzi, F., Pimpinelli, F., Zaninetti, R., Cassetti, A., Coco, S., et al. (2008). Polarization of caveolins and caveolae during migration of immortalized neurons. JOURNAL OF NEUROCHEMISTRY, 104(2), 514-523 [10.1111/j.1471-4159.2007.05005.x].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/5437
Citazioni
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 24
Social impact