Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

Airoldi, C., Tripodi, F., Guzzi, C., Nicastro, R., Coccetti, P. (2015). NMR analysis of budding yeast metabolomics: A rapid method for sample preparation. MOLECULAR BIOSYSTEMS, 11(2), 379-383 [10.1039/c4mb00452c].

NMR analysis of budding yeast metabolomics: A rapid method for sample preparation

AIROLDI, CRISTINA
Primo
;
TRIPODI, FARIDA
Secondo
;
GUZZI, CINZIA;NICASTRO, RAFFAELE;COCCETTI, PAOLA
Ultimo
2015

Abstract

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
Articolo in rivista - Articolo scientifico
NMR spectroscopy, metabolomics, budding yeast
English
2015
11
2
379
383
none
Airoldi, C., Tripodi, F., Guzzi, C., Nicastro, R., Coccetti, P. (2015). NMR analysis of budding yeast metabolomics: A rapid method for sample preparation. MOLECULAR BIOSYSTEMS, 11(2), 379-383 [10.1039/c4mb00452c].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/53917
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