Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
Airoldi, C., Tripodi, F., Guzzi, C., Nicastro, R., Coccetti, P. (2015). NMR analysis of budding yeast metabolomics: A rapid method for sample preparation. MOLECULAR BIOSYSTEMS, 11(2), 379-383 [10.1039/c4mb00452c].
NMR analysis of budding yeast metabolomics: A rapid method for sample preparation
AIROLDI, CRISTINA
Primo
;TRIPODI, FARIDASecondo
;GUZZI, CINZIA;NICASTRO, RAFFAELE;COCCETTI, PAOLA
Ultimo
2015
Abstract
Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.
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