Background: In many areas of Europe, double sensitization to ragweed and mugwort is common, and because of the overlapping flowering periods of the 2 plants, it is not possible to diagnose the primary sensitizing allergen source and hence to determine the proper immunotherapy. Objectives: To elucidate whether double-sensitized patients are cosensitized or cross-sensitized and, in the latter case, to define the primary sensitizer. Methods: Serum samples from 34 patients with late summer respiratory allergy underwent skin prick testing with whole ragweed, and mugwort extracts were analyzed for their reactivity to recombinant Art v 1 and Amb a 1 by ImmunoCAP and then to Amb a 1, Art v 6, and Art v 1 isoforms by a proteomic approach. In double reactors, the primary sensitizing sources were detected by inhibition experiments. Results: Serum samples from patients monosensitized to ragweed contained IgE to epitopes specific of all Amb a 1 isoforms. In contrast, serum samples from double reactors found to be primarily sensitized to mugwort reacted to Art v 1 and Art v 6 and cross-reacted to a few Amb a 1 isoforms. Finally, serum samples from double reactors found to be primarily sensitized to ragweed contained IgE reacting to all Amb a 1 isoforms, part of which cross-reacted to Art v 6. We did not find cosensitized patients. Conclusion: This study found that Art v 6 plays an important role in mugwort allergy and that the cross- reactivity between Art v 6 and Amb a 1 is frequent, bidirectional, and clinically relevant in the area of Milan.

Asero, R., Bellotto, E., Ghiani, A., Aina, R., Villalta, D., Citterio, S. (2014). Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy?. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 113(3), 307-313 [10.1016/j.anai.2014.06.009].

Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy?

BELLOTTO, EMANUELE;GHIANI, ALESSANDRA;AINA, ROBERTA;CITTERIO, SANDRA
2014

Abstract

Background: In many areas of Europe, double sensitization to ragweed and mugwort is common, and because of the overlapping flowering periods of the 2 plants, it is not possible to diagnose the primary sensitizing allergen source and hence to determine the proper immunotherapy. Objectives: To elucidate whether double-sensitized patients are cosensitized or cross-sensitized and, in the latter case, to define the primary sensitizer. Methods: Serum samples from 34 patients with late summer respiratory allergy underwent skin prick testing with whole ragweed, and mugwort extracts were analyzed for their reactivity to recombinant Art v 1 and Amb a 1 by ImmunoCAP and then to Amb a 1, Art v 6, and Art v 1 isoforms by a proteomic approach. In double reactors, the primary sensitizing sources were detected by inhibition experiments. Results: Serum samples from patients monosensitized to ragweed contained IgE to epitopes specific of all Amb a 1 isoforms. In contrast, serum samples from double reactors found to be primarily sensitized to mugwort reacted to Art v 1 and Art v 6 and cross-reacted to a few Amb a 1 isoforms. Finally, serum samples from double reactors found to be primarily sensitized to ragweed contained IgE reacting to all Amb a 1 isoforms, part of which cross-reacted to Art v 6. We did not find cosensitized patients. Conclusion: This study found that Art v 6 plays an important role in mugwort allergy and that the cross- reactivity between Art v 6 and Amb a 1 is frequent, bidirectional, and clinically relevant in the area of Milan.
Articolo in rivista - Articolo scientifico
Amb a 1, Art v 6, ragweed, mugwort
English
2014
113
3
307
313
open
Asero, R., Bellotto, E., Ghiani, A., Aina, R., Villalta, D., Citterio, S. (2014). Concomitant sensitization to ragweed and mugwort pollen: who is who in clinical allergy?. ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 113(3), 307-313 [10.1016/j.anai.2014.06.009].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/53798
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