Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to remedy blood disorders. Here we describe the ex vivo base editing of human CD34+ hematopoietic stem and progenitor cells (HSPCs) by electroporation of base editor mRNA or protein.
Zeng, J., Casirati, G., Nguyen, M., Genovese, P., Bauer, D. (2023). Base Editing of Human Hematopoietic Stem Cells. In S. Bae, B. Song (a cura di), Base Editors Methods and Protocols (pp. 43-62). Humana Press Inc. [10.1007/978-1-0716-2879-9_5].
Base Editing of Human Hematopoietic Stem Cells
Casirati G.;
2023
Abstract
Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to remedy blood disorders. Here we describe the ex vivo base editing of human CD34+ hematopoietic stem and progenitor cells (HSPCs) by electroporation of base editor mRNA or protein.
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