Mps-one-binder (Mob) proteins play an important role in chromosome separation and cell plate formation in yeast. We cloned two Mob1-like genes from alfalfa (Medicago sativa L.) and show that one gene is constitutively expressed while the other is expressed only in flower buds during sporogenesis and gametogenesis. For the analysis of gene expression during reproduction in alfalfa wild-types and apomeiotic mutants, a specific antisense riboprobe was designed for MsMob1 transcripts and a polyclonal antibody was raised against MsMob1 proteins. In situ mRNA localization as well as protein immunolocalization proved that MsMob1-like genes are specifically expressed in degenerating megaspores of normal ovules and in enlarged megaspore mother cells and embryo sacs of apomeiotic ovules. Gene products were also found in microspore tetrads at the beginning of pollen development as well as in tapetum cells of anthers undergoing programmed cell death to allow pollen dispersal at maturity. Overall results suggest that MsMob1-like genes can play a key role during the reproductive pathway in plants.

Citterio, S., Albertini, E., Varotto, S., Feltrin, E., Soattin, M., Marconi, G., et al. (2005). Alfalfa Mob1-like genes are expressed in reproductive organs during meiosis and gametogenesis. PLANT MOLECULAR BIOLOGY, 58(6), 789-807 [10.1007/s11103-005-8104-9].

Alfalfa Mob1-like genes are expressed in reproductive organs during meiosis and gametogenesis

CITTERIO, SANDRA;SGORBATI, SERGIO;
2005

Abstract

Mps-one-binder (Mob) proteins play an important role in chromosome separation and cell plate formation in yeast. We cloned two Mob1-like genes from alfalfa (Medicago sativa L.) and show that one gene is constitutively expressed while the other is expressed only in flower buds during sporogenesis and gametogenesis. For the analysis of gene expression during reproduction in alfalfa wild-types and apomeiotic mutants, a specific antisense riboprobe was designed for MsMob1 transcripts and a polyclonal antibody was raised against MsMob1 proteins. In situ mRNA localization as well as protein immunolocalization proved that MsMob1-like genes are specifically expressed in degenerating megaspores of normal ovules and in enlarged megaspore mother cells and embryo sacs of apomeiotic ovules. Gene products were also found in microspore tetrads at the beginning of pollen development as well as in tapetum cells of anthers undergoing programmed cell death to allow pollen dispersal at maturity. Overall results suggest that MsMob1-like genes can play a key role during the reproductive pathway in plants.
Articolo in rivista - Articolo scientifico
apomeiosis; gametogenesis; Medicago sativa L.; Mps-one-binder; programmed cell death; reproductive mutants
English
2005
58
6
789
807
none
Citterio, S., Albertini, E., Varotto, S., Feltrin, E., Soattin, M., Marconi, G., et al. (2005). Alfalfa Mob1-like genes are expressed in reproductive organs during meiosis and gametogenesis. PLANT MOLECULAR BIOLOGY, 58(6), 789-807 [10.1007/s11103-005-8104-9].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/532
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