In this work we report that budding yeasts carrying human-type telomeric repeats at their chromosome termini show a chronic activation of the Rad53-dependent DNA damage checkpoint pathway and a G2/M cell cycle delay. Furthermore, in the absence of either TEL1/ATM or MEC1/ATR genes, which encodes phosphatidylinositol 3-kinase-related kinases (PIKKs), we detected telomere fusions, whose appearance correlates with a reduced cell viability and a high rate of genome instability. Based on sequence analysis, telomere fusions occurred primarily between ultrashort telomeres. Microcolony formation assays argue against the possibility that fusion-containing cells are eliminated by PIKK-dependent signalling. These findings reveal that humanized telomeres in yeast cells are sensed as a chronically damaged DNA but do not greatly impair cell viability as long as the cells have a functional DNA damage checkpoint.
di Domenico, E.G., Auriche, C., Viscardi, V., Longhese, M.P., Gilson, E., & Ascenzioni, F. (2009). The Mec1p and Tel1p checkpoint kinases allow humanized yeast to tolerate chronic telomere dysfunctions by suppressing telomere fusions. DNA REPAIR, 8(2), 209-218.
Citazione: | di Domenico, E.G., Auriche, C., Viscardi, V., Longhese, M.P., Gilson, E., & Ascenzioni, F. (2009). The Mec1p and Tel1p checkpoint kinases allow humanized yeast to tolerate chronic telomere dysfunctions by suppressing telomere fusions. DNA REPAIR, 8(2), 209-218. |
Tipo: | Articolo in rivista - Articolo scientifico |
Carattere della pubblicazione: | Scientifica |
Titolo: | The Mec1p and Tel1p checkpoint kinases allow humanized yeast to tolerate chronic telomere dysfunctions by suppressing telomere fusions |
Autori: | di Domenico, EG; Auriche, C; Viscardi, V; Longhese, MP; Gilson, E; Ascenzioni, F |
Autori: | |
Data di pubblicazione: | 1-feb-2009 |
Lingua: | English |
Rivista: | DNA REPAIR |
Appare nelle tipologie: | 01 - Articolo su rivista |