Intercellular communication governs multicellular interactions in complex organisms. A variety of mechanisms exist through which cells can communicate, e.g., cell-cell contact, the release of paracrine/autocrine soluble molecules, or the transfer of extracellular vesicles (EVs). EVs are membrane-surrounded structures released by almost all cell types, acting both nearby and distant from their tissue/organ of origin. In the kidney, EVs are potent intercellular messengers released by all urinary system cells and are involved in cell crosstalk, contributing to physiology and pathogene-sis. Moreover, urine is a reservoir of EVs coming from the circulation after crossing the glomerular filtration barrier—or originating in the kidney. Thus, urine represents an alternative source for biomarkers in kidney-related diseases, potentially replacing standard diagnostic techniques, includ-ing kidney biopsy. This review will present an overview of EV biogenesis and classification and the leading procedures for isolating EVs from body fluids. Furthermore, their role in intra-nephron communication and their use as a diagnostic tool for precision medicine in kidney-related disorders will be discussed.

Cricrì, G., Bellucci, L., Montini, G., Collino, F. (2021). Urinary extracellular vesicles: Uncovering the basis of the pathological processes in kidney-related diseases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(12) [10.3390/ijms22126507].

Urinary extracellular vesicles: Uncovering the basis of the pathological processes in kidney-related diseases

Cricrì, G;
2021

Abstract

Intercellular communication governs multicellular interactions in complex organisms. A variety of mechanisms exist through which cells can communicate, e.g., cell-cell contact, the release of paracrine/autocrine soluble molecules, or the transfer of extracellular vesicles (EVs). EVs are membrane-surrounded structures released by almost all cell types, acting both nearby and distant from their tissue/organ of origin. In the kidney, EVs are potent intercellular messengers released by all urinary system cells and are involved in cell crosstalk, contributing to physiology and pathogene-sis. Moreover, urine is a reservoir of EVs coming from the circulation after crossing the glomerular filtration barrier—or originating in the kidney. Thus, urine represents an alternative source for biomarkers in kidney-related diseases, potentially replacing standard diagnostic techniques, includ-ing kidney biopsy. This review will present an overview of EV biogenesis and classification and the leading procedures for isolating EVs from body fluids. Furthermore, their role in intra-nephron communication and their use as a diagnostic tool for precision medicine in kidney-related disorders will be discussed.
Articolo in rivista - Articolo scientifico
Cell-to-cell communication; Extracellular vesicles; Isolation technique; Kidney disease; Urinary biomarkers;
English
2021
22
12
6507
none
Cricrì, G., Bellucci, L., Montini, G., Collino, F. (2021). Urinary extracellular vesicles: Uncovering the basis of the pathological processes in kidney-related diseases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(12) [10.3390/ijms22126507].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/528321
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