We report the synthesis and the characterization of N-(4-aminobutyl)-N- ethyl-isoluminol (ABEI) macrocyclic lactone, an activated ester with an unusual macrocyclic structure, and its use for efficient ABEI conjugation to proteins. Compared to the equivalent reagent normally used for chemiluminescence protein labeling, the ABEI macrocyclic lactone displays improved chemical properties, including stability and reactivity. We show the simple synthesis and the use of ABEI macrocyclic lactone for efficient chemiluminescence labeling of monoclonal antibody mixtures currently used in clinical immunodiagnostic assays for the detection of the HIV p24 antigen in patients. © 2013 Elsevier Ltd. All rights reserved.

Palmioli, A., Crisma, M., Peggion, C., Brusasca, P., Zanin, D., Dal Corso, A., et al. (2013). A new isoluminol reagent for chemiluminescence labeling of proteins. TETRAHEDRON LETTERS, 54(33), 4446-4450 [10.1016/j.tetlet.2013.06.036].

A new isoluminol reagent for chemiluminescence labeling of proteins

PALMIOLI, ALESSANDRO;PERI, FRANCESCO
2013

Abstract

We report the synthesis and the characterization of N-(4-aminobutyl)-N- ethyl-isoluminol (ABEI) macrocyclic lactone, an activated ester with an unusual macrocyclic structure, and its use for efficient ABEI conjugation to proteins. Compared to the equivalent reagent normally used for chemiluminescence protein labeling, the ABEI macrocyclic lactone displays improved chemical properties, including stability and reactivity. We show the simple synthesis and the use of ABEI macrocyclic lactone for efficient chemiluminescence labeling of monoclonal antibody mixtures currently used in clinical immunodiagnostic assays for the detection of the HIV p24 antigen in patients. © 2013 Elsevier Ltd. All rights reserved.
Articolo in rivista - Articolo scientifico
Scientifica
isoluminol, protein labeling, chemoluminescence
English
Palmioli, A., Crisma, M., Peggion, C., Brusasca, P., Zanin, D., Dal Corso, A., et al. (2013). A new isoluminol reagent for chemiluminescence labeling of proteins. TETRAHEDRON LETTERS, 54(33), 4446-4450 [10.1016/j.tetlet.2013.06.036].
Palmioli, A; Crisma, M; Peggion, C; Brusasca, P; Zanin, D; Dal Corso, A; Ingallinella, P; Peri, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/51964
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