Question: Provide a topographical and morphological characterization of post-reperfusion molecular penumbra in the rat stroke model of transient middle cerebral artery (MCA) occlusion Methods: Young adult male Wistar rats (n=25) underwent 90 minutes of transient intraluminal MCA occlusion, followed by 24 hours of reperfusion. Immunohistochemistry for Heat Shock Protein 70 (HSP70) and Microtubule-Associated Protein 2 (MAP2), TUNEL staining and Cresyl violet staining were performed in the same or adjacent brain coronal sections within the entire vascular territory supplied by MCA. Results: Peri-lesional regions showed multiple areas of HSP70 immunostaining of variable extent and localization in the neocortex, mostly in superficial cortical layers. HSP70-positive cells were qualified as neurons (both morphologically and by MAP2 staining) and apparently maintained a tissue organization. We interpreted such regions as ‘mini-penumbras’, likely saved by reperfusion, hypothesis supported by lack of TUNEL staining. ‘Mini-penumbras’ surrounded spots or boundaries of low density marked cells interpreted as ‘mini-cores’, penumbra which has progressed to infarction in spite of reperfusion. Within the ischemic lesion, infarcted cortical and subcortical areas presented a large number of TUNEL-positive cells and a low density of regularly distributed HSP70 marked cells, mainly identified as endothelial cells and rarely as neurons. Conclusions: Qualitative assessment of residual molecular penumbra after 24 hours of reperfusion demonstrated a high variability in staining topography, pattern and extension, along with the emerging concept of heterogeneous penumbra. The preferential localization of “mini-penumbras” in the superficial cortical areas might reflect the variable contribution of leptomeningeal collateral circulation during the ischemic phase.
Cuccione, E., Beretta, S., Padovano, G., Versace, A., Cai, R., Dell'Era, V., et al. (2014). Topographical and morphological patterns of post reperfusion molecular penumbra. In Abstract Book - 8th International Symposium on Neuroprotection and Neurorepair | April 9-12, 2014 | Magdeburg | Germany.
|Citazione:||Cuccione, E., Beretta, S., Padovano, G., Versace, A., Cai, R., Dell'Era, V., et al. (2014). Topographical and morphological patterns of post reperfusion molecular penumbra. In Abstract Book - 8th International Symposium on Neuroprotection and Neurorepair | April 9-12, 2014 | Magdeburg | Germany.|
|Tipo:||abstract + poster|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||No|
|Titolo:||Topographical and morphological patterns of post reperfusion molecular penumbra|
|Autori:||Cuccione, E; Beretta, S; Padovano, G; Versace, A; Cai, R; Dell'Era, V; Rodriguez Menendez, v; Riva, M; Carone, D; Ferrarese, C|
|Data di pubblicazione:||2014|
|Nome del convegno:||International Symposium on Neuroprotection and Neurorepair - April 9-12|
|Appare nelle tipologie:||02 - Intervento a convegno|