NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines. © Springer Science+Business Media New York 2013.
Gelati, M., Profico, D., Projetti Pensi, M., Muzi, G., Sgaravizzi, G., Vescovi, A. (2013). Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system. In B.A. Reynolds, L.P. Deleyrolle (a cura di), Neural Progenitor Cells : Methods and Protocol (pp. 65-77). Springer [10.1007/978-1-62703-574-3-6].
Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system
VESCOVI, ANGELO LUIGI
2013
Abstract
NSCs have been demonstrated to be very useful in grafts into the mammalian central nervous system to investigate the exploitation of NSC for the therapy of neurodegenerative disorders in animal models of neurodegenerative diseases. To push cell therapy in CNS on stage of clinical application, it is necessary to establish a continuous and standardized, clinical grade (i.e., produced following the good manufacturing practice guidelines) human neural stem cell lines. In this chapter, we illustrate some of the protocols routinely used into our GMP cell bank for the production of "clinical grade" human neural stem cell lines. © Springer Science+Business Media New York 2013.
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