Vaccinia virus (VACV) was used for smallpox eradication, but its ultimate origin remains unknown. The genetic relationships among vaccine stocks are also poorly understood. We analyzed 63 vaccine strains with different origin, as well horsepox virus (HPXV). Results indicated the genetic diversity of VACV is intermediate between variola and cowpox viruses, and that mutation contributed more than recombination to VACV evolution. STRUCTURE identified 9 contributing subpopulations and showed that the lowest drift was experienced by the ancestry components of Tian Tan and HPXV/Mütter/Mulford genomes. Subpopulations that experienced very strong drift include those that contributed the ancestry of MVA and IHD-W, in good agreement with the very long passage history of these vaccines. Another highly drifted population contributed the full ancestry of viruses sampled from human/cattle infections in Brazil and, partially, to IOC clones, strongly suggesting that the recurrent infections in Brazil derive from the spillback of IOC to the feral state.

Molteni, C., Forni, D., Cagliani, R., Clerici, M., Sironi, M. (2022). Genetic ancestry and population structure of vaccinia virus. NPJ VACCINES, 7(1) [10.1038/s41541-022-00519-4].

Genetic ancestry and population structure of vaccinia virus

Sironi M
2022

Abstract

Vaccinia virus (VACV) was used for smallpox eradication, but its ultimate origin remains unknown. The genetic relationships among vaccine stocks are also poorly understood. We analyzed 63 vaccine strains with different origin, as well horsepox virus (HPXV). Results indicated the genetic diversity of VACV is intermediate between variola and cowpox viruses, and that mutation contributed more than recombination to VACV evolution. STRUCTURE identified 9 contributing subpopulations and showed that the lowest drift was experienced by the ancestry components of Tian Tan and HPXV/Mütter/Mulford genomes. Subpopulations that experienced very strong drift include those that contributed the ancestry of MVA and IHD-W, in good agreement with the very long passage history of these vaccines. Another highly drifted population contributed the full ancestry of viruses sampled from human/cattle infections in Brazil and, partially, to IOC clones, strongly suggesting that the recurrent infections in Brazil derive from the spillback of IOC to the feral state.
Articolo in rivista - Articolo scientifico
vaccinia vaccine
English
11-ago-2022
2022
7
1
92
open
Molteni, C., Forni, D., Cagliani, R., Clerici, M., Sironi, M. (2022). Genetic ancestry and population structure of vaccinia virus. NPJ VACCINES, 7(1) [10.1038/s41541-022-00519-4].
File in questo prodotto:
File Dimensione Formato  
Molteni-2022-npj Vaccines-VoR.pdf

accesso aperto

Descrizione: s This article is licensed under a Creative Commons Attribution 4.0 International License
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 2.53 MB
Formato Adobe PDF
2.53 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/509819
Citazioni
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 10
Social impact