Despite the relevance of carbohydrates as cues in eliciting specific biological responses, the covalent surface modification of collagen-based matrices with small carbohydrate epitopes has been scarcely investigated. We report thereby the development of an efficient procedure for the chemoselective neoglycosylation of collagen matrices (patches) via a thiol-ene approach, between alkene-derived monosaccharides and the thiol-functionalized material surface. SR-XPS, FTIR and ELLA assays confirmed the effectiveness of the collagen neoglycosylation. Preliminary biological evaluation in osteoarthritic models is reported. The proposed methodology can be extended to any thiolated surface for the development of smart biomaterials for innovative approaches in regenerative medicine.

Russo, L., Battocchio, C., Secchi, V., Magnano, E., Nappini, S., Taraballi, F., et al. (2014). Thiol–ene Mediated Neoglycosylation of Collagen Patches: A Preliminary Study. LANGMUIR, 30(5), 1336-1342 [10.1021/la404310p].

Thiol–ene Mediated Neoglycosylation of Collagen Patches: A Preliminary Study

RUSSO, LAURA;TARABALLI, FRANCESCA;GABRIELLI, LUCA;COMELLI, FRANCESCA;PAPAGNI, ANTONIO;COSTA, BARBARA SIMONA;NICOTRA, FRANCESCO;NATALELLO, ANTONINO;DOGLIA, SILVIA MARIA;CIPOLLA, LAURA FRANCESCA
2014

Abstract

Despite the relevance of carbohydrates as cues in eliciting specific biological responses, the covalent surface modification of collagen-based matrices with small carbohydrate epitopes has been scarcely investigated. We report thereby the development of an efficient procedure for the chemoselective neoglycosylation of collagen matrices (patches) via a thiol-ene approach, between alkene-derived monosaccharides and the thiol-functionalized material surface. SR-XPS, FTIR and ELLA assays confirmed the effectiveness of the collagen neoglycosylation. Preliminary biological evaluation in osteoarthritic models is reported. The proposed methodology can be extended to any thiolated surface for the development of smart biomaterials for innovative approaches in regenerative medicine.
Articolo in rivista - Articolo scientifico
Scientifica
carbohydrates, thiol-ene reaction, SR-induced X-ray Photoelectron Spectroscopy, Bioorganic chemistry, collagen
English
Russo, L., Battocchio, C., Secchi, V., Magnano, E., Nappini, S., Taraballi, F., et al. (2014). Thiol–ene Mediated Neoglycosylation of Collagen Patches: A Preliminary Study. LANGMUIR, 30(5), 1336-1342 [10.1021/la404310p].
Russo, L; Battocchio, C; Secchi, V; Magnano, E; Nappini, S; Taraballi, F; Gabrielli, L; Comelli, F; Papagni, A; Costa, B; Polzonetti, G; Nicotra, F; Natalello, A; Doglia, S; Cipolla, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/50643
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