We perform time-correlated single-photon counting measurements with 30-ps resolution of the fluorescence emitted by the donor fluorophores of donor-acceptor pairs covalently labeling DNA synthetic oligonucleotides by using a non-commercial single-photon avalanche diode. The measurements allow us to precisely assess the fluorescence resonant energy transfer efficiency of the pairs in solutions containing the oligonucleotides and DNA-ligands, for different ratios of the DNA base-pair to ligand concentrations. A quantitative evaluation of the deformations of DNA double strands following the ligand binding is obtained, as the transfer efficiency is a steep function of the donor-to-acceptor distance. The results lead to an easy and cheap method to discriminate between the binding modes of minor groove and base intercalating ligands. © 2009 - IOS Press. All rights reserved.

Nardo, L., Bondani, M., Andreoni, A. (2009). Discrimination of the binding mode of DNA ligands by single-photon timing. SPECTROSCOPY, 23(1), 11-28 [10.3233/SPE-2009-0370].

Discrimination of the binding mode of DNA ligands by single-photon timing

NARDO, LUCA;
2009

Abstract

We perform time-correlated single-photon counting measurements with 30-ps resolution of the fluorescence emitted by the donor fluorophores of donor-acceptor pairs covalently labeling DNA synthetic oligonucleotides by using a non-commercial single-photon avalanche diode. The measurements allow us to precisely assess the fluorescence resonant energy transfer efficiency of the pairs in solutions containing the oligonucleotides and DNA-ligands, for different ratios of the DNA base-pair to ligand concentrations. A quantitative evaluation of the deformations of DNA double strands following the ligand binding is obtained, as the transfer efficiency is a steep function of the donor-to-acceptor distance. The results lead to an easy and cheap method to discriminate between the binding modes of minor groove and base intercalating ligands. © 2009 - IOS Press. All rights reserved.
Articolo in rivista - Articolo scientifico
Drug-DNA binding; intercalation; Groove binding; time-resolved FRET
English
2009
23
1
11
28
none
Nardo, L., Bondani, M., Andreoni, A. (2009). Discrimination of the binding mode of DNA ligands by single-photon timing. SPECTROSCOPY, 23(1), 11-28 [10.3233/SPE-2009-0370].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/49094
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