: The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose-dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.

Braam, S., Tripodi, F., Österberg, L., Persson, S., Welkenhuysen, N., Coccetti, P., et al. (2024). Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches. MICROBIAL CELL, 11, 143-154 [10.15698/mic2024.05.822].

Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches

Tripodi, Farida
Secondo
;
Coccetti, Paola
Penultimo
;
2024

Abstract

: The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose-dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.
Articolo in rivista - Articolo scientifico
Snf1/Mig1 network; fluorescence recovery after photobleaching; nonlinear mixed effect model; nuclear localization; nutrient signaling; yeast
English
2024
11
143
154
open
Braam, S., Tripodi, F., Österberg, L., Persson, S., Welkenhuysen, N., Coccetti, P., et al. (2024). Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches. MICROBIAL CELL, 11, 143-154 [10.15698/mic2024.05.822].
File in questo prodotto:
File Dimensione Formato  
Braam-2024-MiC-VoR.pdf

accesso aperto

Descrizione: Braam 2024
Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 2.51 MB
Formato Adobe PDF
2.51 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/484779
Citazioni
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
Social impact