The muscle-specific variant of neuronal nitric oxide (NO) synthase (NOS-I), is developmentally regulated in mouse suggesting a role of NO during myogenesis. In chick embryo, a good model of development, we found that the expression of NOS-I is up-regulated, but only in the early phase of development. Through a pharmacological intervention in ovo we found that NO signalling plays a relevant role during embryonic development. The inhibition of NOS-I decreased the growth of embryo, in particular of muscle tissue, while the restoring of physiological NO levels, via administration of a NO donor, reversed this effect. We found a selective action of NO, produced by NOS-I, on regulatory factors involved in myogenic differentiation in the early phase of chick embryo development: inhibition of NO generation leads to a decreased expression of the Myocyte enhancer factor 2a (Mef2a), Mef2c, Myogenin and Myosin, which was reversed by the administration of a NO donor. NO had no effects on Myf5 and MyoD, the myogenic regulatory factors necessary for myogenic determination. The action of NO on the myogenic regulatory factors was mediated via generation of cyclic GMP (cGMP) and activation of the cGMP-dependent protein kinase G (PKG). Finally we found in myoblasts in vitro that the activation of Mef2c was the key event mediating the NO-induced modulation of myogenesis. Our results identify NO produced by NOS-I as a key messenger in the early phase of embryonic development of chicken, acting as a critical determinant of myogenesis through its physiological cGMP/PKG pathway.

Cazzato, D., Assi, E., Moscheni, C., Brunelli, S., De Palma, C., Cervia, D., et al. (2014). Nitric Oxide drives embryonic myogenesis in chicken through the upregulation of myogenic differentiation factors. EXPERIMENTAL CELL RESEARCH, 320(2), 269-280 [10.1016/j.yexcr.2013.11.006].

Nitric Oxide drives embryonic myogenesis in chicken through the upregulation of myogenic differentiation factors

BRUNELLI, SILVIA;
2014

Abstract

The muscle-specific variant of neuronal nitric oxide (NO) synthase (NOS-I), is developmentally regulated in mouse suggesting a role of NO during myogenesis. In chick embryo, a good model of development, we found that the expression of NOS-I is up-regulated, but only in the early phase of development. Through a pharmacological intervention in ovo we found that NO signalling plays a relevant role during embryonic development. The inhibition of NOS-I decreased the growth of embryo, in particular of muscle tissue, while the restoring of physiological NO levels, via administration of a NO donor, reversed this effect. We found a selective action of NO, produced by NOS-I, on regulatory factors involved in myogenic differentiation in the early phase of chick embryo development: inhibition of NO generation leads to a decreased expression of the Myocyte enhancer factor 2a (Mef2a), Mef2c, Myogenin and Myosin, which was reversed by the administration of a NO donor. NO had no effects on Myf5 and MyoD, the myogenic regulatory factors necessary for myogenic determination. The action of NO on the myogenic regulatory factors was mediated via generation of cyclic GMP (cGMP) and activation of the cGMP-dependent protein kinase G (PKG). Finally we found in myoblasts in vitro that the activation of Mef2c was the key event mediating the NO-induced modulation of myogenesis. Our results identify NO produced by NOS-I as a key messenger in the early phase of embryonic development of chicken, acting as a critical determinant of myogenesis through its physiological cGMP/PKG pathway.
Articolo in rivista - Articolo scientifico
sodium dodecyl sulphate; NO synthases; HH; 1H-[1,2,4]Oxadiazolo[4,3–a]quinoxalin-1-one; horseradish-peroxidase; cyclic GMP; neuronal NOS; cGMP; DETA; NO; PKG; PBS; NOS; glucose 6 phosphate dehydrogenase; 1-[N-(2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate, DMEM, Dulbecco’s Modified Eagle’s Medium; Hamburger and Hamilton; MRFs; GAPDH; NPLA; Chick embryo; Muscle development; N(ω)-Propyl-L-Arginine; soluble guanylate cyclase; FBS; cGMP/PKG; Murine myoblasts; sGC; phosphate buffered saline; HRP; Nitric Oxide; SDS; NOS-I; Mef2; Myocyte enhancer factor 2; myogenic regulatory factors; foetal bovine serum; cGMP-dependent protein kinase; Mef2c; ODQ
English
14-nov-2013
2014
320
2
269
280
none
Cazzato, D., Assi, E., Moscheni, C., Brunelli, S., De Palma, C., Cervia, D., et al. (2014). Nitric Oxide drives embryonic myogenesis in chicken through the upregulation of myogenic differentiation factors. EXPERIMENTAL CELL RESEARCH, 320(2), 269-280 [10.1016/j.yexcr.2013.11.006].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/48194
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