The application of liposomes (LPs) to central nervous system disorders could represents a turning point in the therapy and quality of life of patients. Indeed, LPs have demonstrated their ability to cross the blood-brain barrier (BBB) and, as a consequence, to enhance the therapeutics delivery into the brain. Some approaches for BBB crossing involve the modification of LP surfaces with biologically active ligands. Among them, the Apolipoprotein E-modified peptide (mApoE) has been used for several LP-based nanovectors under investigation. In this study, we propose Surface Plasmon Resonance imaging (SPRi) for the characterization of multifunctionalized LPs for Glioblastoma treatment. LPs were functionalized with mApoE and with a metallo-protease sensitive lipopeptide to deliver and guarantee the localized release of an encapsulated drug in diseased areas. The SPRi analysis was optimized in order to evaluate the binding affinity between LPs and mApoE receptors, finding that mApoE-LPs generated SPRi signals referred to interactions between mApoE and receptors mainly present in the brain. Moreover, a significant binding between LPs and VCAM-1 (endothelial receptor) was observed, whereas LPs did not interact significantly with peripheral receptors expressed on monocytes and lymphocytes. SPRi results confirmed not only the presence of mApoE on LP surfaces, but also its binding affinity, thanks to the specific interaction with selected receptors. In conclusion, the high sensitivity and the multiplexing capability associated with the low volumes of sample required and the minimal sample preparation, make SPRi an excellent technique for the characterization of multifunctionalized nanoparticles-based formulations.The SPRi analysis was optimized to study the interactions between mApoE-functionalized liposomes and receptors present in the brain and on monocytes and lymphocytes, demonstrating to be an excellent technique for characterization of liposomes.

Picciolini, S., Rodà, F., Gualerzi, A., Mangolini, V., Forleo, L., Mangolini, A., et al. (2023). SPRi analysis of molecular interactions of mApoE-functionalized liposomes as drug delivery systems for brain diseases. ANALYST, 148(23), 6070-6077 [10.1039/d3an01507f].

SPRi analysis of molecular interactions of mApoE-functionalized liposomes as drug delivery systems for brain diseases

Sesana S.;Re F.;
2023

Abstract

The application of liposomes (LPs) to central nervous system disorders could represents a turning point in the therapy and quality of life of patients. Indeed, LPs have demonstrated their ability to cross the blood-brain barrier (BBB) and, as a consequence, to enhance the therapeutics delivery into the brain. Some approaches for BBB crossing involve the modification of LP surfaces with biologically active ligands. Among them, the Apolipoprotein E-modified peptide (mApoE) has been used for several LP-based nanovectors under investigation. In this study, we propose Surface Plasmon Resonance imaging (SPRi) for the characterization of multifunctionalized LPs for Glioblastoma treatment. LPs were functionalized with mApoE and with a metallo-protease sensitive lipopeptide to deliver and guarantee the localized release of an encapsulated drug in diseased areas. The SPRi analysis was optimized in order to evaluate the binding affinity between LPs and mApoE receptors, finding that mApoE-LPs generated SPRi signals referred to interactions between mApoE and receptors mainly present in the brain. Moreover, a significant binding between LPs and VCAM-1 (endothelial receptor) was observed, whereas LPs did not interact significantly with peripheral receptors expressed on monocytes and lymphocytes. SPRi results confirmed not only the presence of mApoE on LP surfaces, but also its binding affinity, thanks to the specific interaction with selected receptors. In conclusion, the high sensitivity and the multiplexing capability associated with the low volumes of sample required and the minimal sample preparation, make SPRi an excellent technique for the characterization of multifunctionalized nanoparticles-based formulations.The SPRi analysis was optimized to study the interactions between mApoE-functionalized liposomes and receptors present in the brain and on monocytes and lymphocytes, demonstrating to be an excellent technique for characterization of liposomes.
Articolo in rivista - Articolo scientifico
liposome; drug delivery; blood brain barrier; glioblastoma; SPRi analysis
English
31-ott-2023
2023
148
23
6070
6077
reserved
Picciolini, S., Rodà, F., Gualerzi, A., Mangolini, V., Forleo, L., Mangolini, A., et al. (2023). SPRi analysis of molecular interactions of mApoE-functionalized liposomes as drug delivery systems for brain diseases. ANALYST, 148(23), 6070-6077 [10.1039/d3an01507f].
File in questo prodotto:
File Dimensione Formato  
Picciolini-2023-Analyst-VoR.pdf

Solo gestori archivio

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Tutti i diritti riservati
Dimensione 996.07 kB
Formato Adobe PDF
996.07 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/476481
Citazioni
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
Social impact