In recent decades, biotechnological drugs have emerged as relevant therapeutic tools. However, therapeutic molecules can exert their activity only if properly formulated and delivered into the body. In this regard, nano-sized drug delivery systems have been shown to provide protection, stability, and controlled release of payloads, increasing their therapeutic efficacy. In this work, a microfluidic mixing technique for the preparation of chitosan-based nanoparticles was established with the capability of easily exchanging macromolecular biological cargos such as the model protein β-Galactosidase, mRNA, and siRNA. The nanoparticles obtained showed hydrodynamic diameters ranging from 75 nm to 105 nm, low polydispersity of 0.15 to 0.22 and positive zeta potentials of 6 mV to 17 mV. All payloads were efficiently encapsulated (>80 %) and the well-known cytocompatibility of chitosan-based nanoparticles was confirmed. Cell culture studies demonstrated increased cellular internalization of loaded nano-formulations compared to free molecules as well as successful gene silencing with nano-formulated siRNA, suggesting the ability of these nanoparticles to escape the endosome.

Greco, A., Gabold, B., Chen, S., Wang, X., Xu, Z., Hartschuh, A., et al. (2023). Microfluidic Mixing as Platform Technology for Production of Chitosan Nanoparticles Loaded with Different Macromolecules. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 188, 170-181 [10.1016/j.ejpb.2023.05.010].

Microfluidic Mixing as Platform Technology for Production of Chitosan Nanoparticles Loaded with Different Macromolecules

Greco, A
Primo
;
2023

Abstract

In recent decades, biotechnological drugs have emerged as relevant therapeutic tools. However, therapeutic molecules can exert their activity only if properly formulated and delivered into the body. In this regard, nano-sized drug delivery systems have been shown to provide protection, stability, and controlled release of payloads, increasing their therapeutic efficacy. In this work, a microfluidic mixing technique for the preparation of chitosan-based nanoparticles was established with the capability of easily exchanging macromolecular biological cargos such as the model protein β-Galactosidase, mRNA, and siRNA. The nanoparticles obtained showed hydrodynamic diameters ranging from 75 nm to 105 nm, low polydispersity of 0.15 to 0.22 and positive zeta potentials of 6 mV to 17 mV. All payloads were efficiently encapsulated (>80 %) and the well-known cytocompatibility of chitosan-based nanoparticles was confirmed. Cell culture studies demonstrated increased cellular internalization of loaded nano-formulations compared to free molecules as well as successful gene silencing with nano-formulated siRNA, suggesting the ability of these nanoparticles to escape the endosome.
Articolo in rivista - Articolo scientifico
Chitosan; Drug delivery systems; Microfluidic; mRNA; Nanoplatforms; Protein; siRNA;
English
mag-2022
2023
188
170
181
reserved
Greco, A., Gabold, B., Chen, S., Wang, X., Xu, Z., Hartschuh, A., et al. (2023). Microfluidic Mixing as Platform Technology for Production of Chitosan Nanoparticles Loaded with Different Macromolecules. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 188, 170-181 [10.1016/j.ejpb.2023.05.010].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/475619
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