1,2-Dihydroxynaphthalenes are produced by dehydrogenation of the corresponding 1,2-dihydro-1,2-dihydroxynaphthalenes using an Escherichia coli recombinant strain containing the dihydrodiol naphthalene dehydrogenase gene cloned from Pseudomonas fluorescens N3. Conversions are led in carefully controlled conditions to minimise product polymerisation. A multistep procedure using a weakly basic resin permits isolation of good product amounts, solving the toxicity problem. Products are isolated and characterised as t-butyldimethylsilyl derivatives that are stable compounds. The transformation of the 1,2-dihydroxynaphthalenes into the corresponding 1,2-naphthoquinones is also reported
Cavallotti, C., Orsini, F., Sello, G., Di Gennaro, P., Galli, E., & Bestetti, G. (1999). 1,2-Dihydro-1,2-dihydroxynaphthalene dehydrogenase containing recombinant strains: Preparation, isolation and characterisation of 1,2- dihydroxynaphthalenes and 1,2-naphthoquinones. TETRAHEDRON, 55(14), 4467-4480 [10.1016/S0040-4020(99)00135-0].
Citazione: | Cavallotti, C., Orsini, F., Sello, G., Di Gennaro, P., Galli, E., & Bestetti, G. (1999). 1,2-Dihydro-1,2-dihydroxynaphthalene dehydrogenase containing recombinant strains: Preparation, isolation and characterisation of 1,2- dihydroxynaphthalenes and 1,2-naphthoquinones. TETRAHEDRON, 55(14), 4467-4480 [10.1016/S0040-4020(99)00135-0]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Titolo: | 1,2-Dihydro-1,2-dihydroxynaphthalene dehydrogenase containing recombinant strains: Preparation, isolation and characterisation of 1,2- dihydroxynaphthalenes and 1,2-naphthoquinones | |
Autori: | Cavallotti, C; Orsini, F; Sello, G; Di Gennaro, P; Galli, E; Bestetti, G | |
Autori: | ||
Data di pubblicazione: | 1999 | |
Lingua: | English | |
Rivista: | TETRAHEDRON | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0040-4020(99)00135-0 | |
Appare nelle tipologie: | 01 - Articolo su rivista |