InExpose and vivoFlow system: advances in Refinement and Reduction

In last decades, scientific research is doing its utmost to implement and achieve the ethical principles of the 3Rs. In this respect, our state-of-the-art platform inExpose system (SCIreQ, Emka Technologies) is a radiant example. The latter is a compact exposure system of precision inhalation in nose-only mode, whose computer-controlled nebulizers allow automated generation of aerosols with a precise concentration in the order of seconds. Moreover, our modular platform is combined with vivoFlow system (SCIreQ, Emka Technologies), that allows a whole-body mode inhalation and a contemporary recording of rodents plethysmographic values. Our combined platform offers the great opportunity to performing small-scale studies, evaluating small animal numbers in precise controlled conditions. Thus, this system allows us to study the effect of multiple nanomaterials on murine models thought the respiratory delivery in the entire body with repeatable and non-invasive exposures. Moreover, we have the possibility not only of minimising any animal distress and pain, but also of monitoring the animal welfare during the entire exposure procedures with a better reliability and repeatability of scientific results. In conclusion, this innovative nanotechnological platform is open to the contribution of research groups united by the aim of identifying and validating new therapeutic approaches for diseases, though the development and refinement of experimental models and the study of the efficacy and safety of new drugs.