We report new methods of 2 photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.

Nardini, A., Vazquez, R., Marini, M., Conci, C., Bouzin, M., Collini, M., et al. (2023). Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples. In Proceedings of SPIE - The International Society for Optical Engineering. SPIE [10.1117/12.2672894].

Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples

Marini M.;Bouzin M.;Collini M.;Chirico G.;
2023

Abstract

We report new methods of 2 photon polymerization of microlenses with high numerical aperture, large diameter and good optical quality. We characterize the aberrations of these lenses that, coupled to raster scanning optical microscopes, allow two-photon excitation imaging of cells. In-vivo non-linear imaging will be also discussed, opening the possibility to use these micro-lenses in implants for the continuous inspection of biological dynamics in vivo.
paper
2-photon polymerization; label free imaging; micro-lenses; non-linear excitation microscopy; optical microscopy;
English
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI 2023 - 26 June 2023 through 29 June 2023
2023
Ferraro, P; Psaltis, D; Grilli, S
Proceedings of SPIE - The International Society for Optical Engineering
9781510664531
2023
12622
1262206
none
Nardini, A., Vazquez, R., Marini, M., Conci, C., Bouzin, M., Collini, M., et al. (2023). Two-photon laser polymerized microlenses for non-linear excitation microscopy of biological samples. In Proceedings of SPIE - The International Society for Optical Engineering. SPIE [10.1117/12.2672894].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/466052
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