Cisplatin (CDDP) is cytotoxic, inducing apoptosis in some tumoral cell lines in vitro. Since CDDP is an effective drug in vivo treatment of neuroblastoma, we tested this drug on the human neuroblastoma SH-SY5Y cell line. Materials and methods. The effect of CDDP on cultured SH-SY5Y cells was determined with trypan blue dye exclusion test, LDH activity and DNA electrophoresis. Cultures were observed by light and electron microscope. Flow cytometric analysis was also carried out.

Cece, R., Barajon, I., Tredici, G. (1995). Cisplatin induces apoptosis in SH-SY5Y human neuroblastoma cell line. ANTICANCER RESEARCH, 15(3), 777-782.

Cisplatin induces apoptosis in SH-SY5Y human neuroblastoma cell line

TREDICI, GIOVANNI
1995

Abstract

Cisplatin (CDDP) is cytotoxic, inducing apoptosis in some tumoral cell lines in vitro. Since CDDP is an effective drug in vivo treatment of neuroblastoma, we tested this drug on the human neuroblastoma SH-SY5Y cell line. Materials and methods. The effect of CDDP on cultured SH-SY5Y cells was determined with trypan blue dye exclusion test, LDH activity and DNA electrophoresis. Cultures were observed by light and electron microscope. Flow cytometric analysis was also carried out.
Articolo in rivista - Articolo scientifico
Trypan Blue; Apoptosis; Chromatin; Dose-Response Relationship, Drug; Humans; Neuroblastoma; DNA, Neoplasm; Tumor Cells, Cultured; Cisplatin; Cytoplasm; Kinetics; Microscopy, Electron; Time Factors; Cell Line
English
1995
15
3
777
782
none
Cece, R., Barajon, I., Tredici, G. (1995). Cisplatin induces apoptosis in SH-SY5Y human neuroblastoma cell line. ANTICANCER RESEARCH, 15(3), 777-782.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/45702
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