Organic electrochemical transistors (OECTs) have shown great potential in bioelectronics to transduce small biological signals for applications such as the electrical recording of excitable cells and assessing cell barrier properties. It is imperative that operating the OECT as a biosensor does not affect the biological system. However, bias voltages applied to channel materials such as the conducting polymer (CP) PEDOT:PSS have been shown to induce the formation of hydrogen peroxide (H2O2) which can disrupt the physiology of cells. In this work, we evaluated the impact of H2O2 formation during OECT operation by comparing an oxygen-sensitive CP (PEDOT:PSS) and an oxygen-stable CP (p(gPyDPP-MeOT2)). While both CPs show high biocompatibility in their non-biased, resting state, we observed large differences during the operation of the electrochemical device. OECTs with PEDOT:PSS produce H2O2 where the H2O2 concentration in the electrolyte depends on the channel area and the time of operation. In comparison, OECTs using the oxygen-stable DPP-based polymer showed no sign of H2O2 formation. Further investigation also revealed how the proliferation rate of neuronal cells directly interfaced with such OECTs was affected by the concentration of H2O2. Our work demonstrated the limitations of oxygen-sensitive OECT channel materials for bioelectronic applications and provides guidance for material design strategies to develop safe bioelectronic devices for real-life applications.

Lubrano, C., Bettucci, O., Dijk, G., Salleo, A., Giovannitti, A., Santoro, F. (2024). The impact of hydrogen peroxide production in OECTs for in vitro applications. JOURNAL OF MATERIALS CHEMISTRY. C, 12(5 (07 February 2024)), 1625-1630 [10.1039/D3TC02849F].

The impact of hydrogen peroxide production in OECTs for in vitro applications

Bettucci, Ottavia
Co-primo
;
2024

Abstract

Organic electrochemical transistors (OECTs) have shown great potential in bioelectronics to transduce small biological signals for applications such as the electrical recording of excitable cells and assessing cell barrier properties. It is imperative that operating the OECT as a biosensor does not affect the biological system. However, bias voltages applied to channel materials such as the conducting polymer (CP) PEDOT:PSS have been shown to induce the formation of hydrogen peroxide (H2O2) which can disrupt the physiology of cells. In this work, we evaluated the impact of H2O2 formation during OECT operation by comparing an oxygen-sensitive CP (PEDOT:PSS) and an oxygen-stable CP (p(gPyDPP-MeOT2)). While both CPs show high biocompatibility in their non-biased, resting state, we observed large differences during the operation of the electrochemical device. OECTs with PEDOT:PSS produce H2O2 where the H2O2 concentration in the electrolyte depends on the channel area and the time of operation. In comparison, OECTs using the oxygen-stable DPP-based polymer showed no sign of H2O2 formation. Further investigation also revealed how the proliferation rate of neuronal cells directly interfaced with such OECTs was affected by the concentration of H2O2. Our work demonstrated the limitations of oxygen-sensitive OECT channel materials for bioelectronic applications and provides guidance for material design strategies to develop safe bioelectronic devices for real-life applications.
Articolo in rivista - Articolo scientifico
Conductive polymers; OECTs; Bioelectronics
English
20-dic-2023
2024
12
5 (07 February 2024)
1625
1630
open
Lubrano, C., Bettucci, O., Dijk, G., Salleo, A., Giovannitti, A., Santoro, F. (2024). The impact of hydrogen peroxide production in OECTs for in vitro applications. JOURNAL OF MATERIALS CHEMISTRY. C, 12(5 (07 February 2024)), 1625-1630 [10.1039/D3TC02849F].
File in questo prodotto:
File Dimensione Formato  
Lubrano-2024-J Mater Chem C-AAM.pdf

accesso aperto

Tipologia di allegato: Author’s Accepted Manuscript, AAM (Post-print)
Licenza: Creative Commons
Dimensione 1.71 MB
Formato Adobe PDF
1.71 MB Adobe PDF Visualizza/Apri
10281-455853_VoR.pdf

accesso aperto

Tipologia di allegato: Publisher’s Version (Version of Record, VoR)
Licenza: Creative Commons
Dimensione 1.19 MB
Formato Adobe PDF
1.19 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/455853
Citazioni
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
Social impact